# CYTOSKELETAL HYBRIDOMA ANTIBODIES AS DIAGNOSTIC REAGENTS

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1988 · $173,263

## Abstract

The goal of this project is to generate monoclonal antibodies to different
cytoskeletal proteins and use these antibodies as tissue-specific reagents
in diagnostic surgical pathology.  The cytoskeleton (particularly the
intermediate filaments) of different cells is composed of different
proteins, and antibodies capable of recognizing specific proteins are
therefore useful as tissue-specific reagents.  We intend to generate a
panel of such hybridomas capable of recognizing different cells and use
them to accurately determine the cellular nature of poorly differentiated
neoplasms.  Cytoskeleton from different normal and malignant human cells
such as lymphocytes, lymphomas, melanomas, different carcinomas, different
sarcomas, and neural tumors will be used as immunogens.  Antibodies will be
selected for their ability to react specifically with one cell type and not
with others.  Antigens recognized by the different antibodies will be
determined by Western blot experiments.

The different antibodies will be used to study the cell biology of the
different cytoskeletal proteins.  We will localize the different
intermediate filament proteins in different cells by immunoelectron
microscopy, asking if the different proteins are found in similar filaments
or if they polymerize into homogeneous filaments.  The protein composition
of tonofilament bundles and desmosomes will also be investigated.  Attempts
at sequencing fragments of the different intermediate filament proteins
will be undertaken by fragmenting the different proteins with proteases or
cyanogen bromide and isolating fragments that retain the ability to react
with the different antibodies.  The amino acid sequence of these fragments
will be determined.  These studies should identify subtle differences in
intermediate filament proteins, which then can be used to generate
additional antibodies with greater specificity.  (4)

## Key facts

- **NIH application ID:** 3173789
- **Project number:** 5R01CA036250-05
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** ALLEN M. GOWN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $173,263
- **Award type:** 5
- **Project period:** 1984-03-01 → 1992-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173789

## Citation

> US National Institutes of Health, RePORTER application 3173789, CYTOSKELETAL HYBRIDOMA ANTIBODIES AS DIAGNOSTIC REAGENTS (5R01CA036250-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173789. Licensed CC0.

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