# STEROIDS IN TUMOR GROWTH OF THE MALE REPRODUCTIVE TRACT

> **NIH NIH R01** · BAYLOR COLLEGE OF MEDICINE · 1988 · $136,024

## Abstract

The long term goal of this proposal is to understand how to
control growth of hormone responsive tumors of the male
reproductive tract.

Two hypotheses are to be tested.  the first is that androgens via
their receptors stimulate growth by inducing a cascade process
which results in the production and/or stabilization of transcripts
coding for competence factors; this process can be modified by
other regulatory agents such as glucocorticoids, which function
via their receptors to decrease the half-life of these androgen
induced mRNA's thus maintaining the cells in a G1/G0 or a state.
The first hypothesis will be investigated by measuring the
concentrations of c-sis, c-fos and c-myc transcriptts as a function
of time following testosterone (T) or dihydrotestosterone
treatment and investigating the effects of cycloheximide on this
process.  The time required to establish steady-state levels of sis
transcripts will be measure following androgen treatment and
then the time required to reach a new steady state following
addition of the glucocorticoid triamcinolone acetonide TA) will be
determined.  After having quantitated the effects of androgens
and glucocorticoids on sis, the RNA will be reassayed with a probe
for 2'-5'-oligo-A synthetase to determine whether alterations in
its expression is associated with changes in concentration of sis
transcripts.  It will next be determined whether the opposing
effects of T and TA on the levels of sis transcripts is explained by
changes in half-life of the mRNA and/or alterations regions which
are present of the 4.3 kb c-sis transcripts, are involved in
glucocorticoid regulatory events by transfection experiments
utilizing R3327H-G8-A1 rat prostate cancer cells.  In the
R3327H-G8-A1 cell line and rat prostate will determine whether
reductions in epidermal growth factor (EGF), transforming growth
factor alpha (TGF), EGF receptor and c-sis transcripts accompany
the glucocorticoid inhibitory effect of growth.

Finally, because there appears to be an association with increased
expression of c-sis in human BPH and prostate cancer we will test
our second hypothesis that abnormal expression of sis is related to
abnormal prostatic growth.  This will be investigated by producing
transgenic mice where the sis gene is placed adjacent to an
androgen receptor inducible promoter element.

## Key facts

- **NIH application ID:** 3173809
- **Project number:** 5R01CA036264-05
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **SAM.gov UEI:** FXKMA43NTV21
- **Principal Investigator:** Dolores Jean Lamb
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $136,024
- **Award type:** 5
- **Project period:** 1984-01-01 → 1989-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173809

## Citation

> US National Institutes of Health, RePORTER application 3173809, STEROIDS IN TUMOR GROWTH OF THE MALE REPRODUCTIVE TRACT (5R01CA036264-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173809. Licensed CC0.

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