# MONOKINES IN HEMATOPOIESIS

> **NIH NIH R01** · OREGON HEALTH & SCIENCE UNIVERSITY · 1988 · $170,200

## Abstract

Mononuclear phagocytes (M) are important hematopoietic
regulatory cells.  Our studies indicate that the granulopoietic
activity of M invitro are mediated through other auxiliary cells,
including endothelial cells (E) and fibroblasts (F), both
constituents of the hematopoietic microenvironment.  We have
described a model in which, M produce monokine (MRA) which
stimulates the production and release of CSA by marrow stromal
cells.  In the past 2year funding period we have determined; 1)
that MRA is interleukin1 (IL1), 2) that similar monokines induce
the production and release of growth factors for erythroid bursts,
megakaryocytic CFU, and pluripotent -CFU (CFUGEMM) by
endothelial cells and marrow fibroblasts, and 3) that autonomous
granulopoieses in-vitro is a unique characteristic of chronic
granulocytic leukemia of the juvenile type (JCGL) and that this
pattern of growth results from excessive activity of
moncytederived IL1.  During this period we have also developed
a technique by which gene expression can be studied at various
stages of normal human granulopoietic cellular differentiation.
We propose to:  1) characterize coordinately expressed IL1
induced genes by stromal cells, 2) identify cofactors for IL-1
production and IL-1 responsiveness, 3) identify the breadth of
growth factors induced by IL-1, 4) identify the differentiation
stages at which daughters of CFUGM become competent for
IL1 gene expression, and 5) characterize molecular defects of
IL1 gene expression in children with JCGL.  These studies may
identify molecular mechanisms by which stromal cells of the
marrow interact with moncyte/macrophages to regulate normal
and leukemic hematopoiesis.

## Key facts

- **NIH application ID:** 3173852
- **Project number:** 5R01CA036306-05
- **Recipient organization:** OREGON HEALTH & SCIENCE UNIVERSITY
- **SAM.gov UEI:** NPSNT86JKN51
- **Principal Investigator:** Grover Carlton Bagby
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $170,200
- **Award type:** 5
- **Project period:** 1984-01-01 → 1991-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173852

## Citation

> US National Institutes of Health, RePORTER application 3173852, MONOKINES IN HEMATOPOIESIS (5R01CA036306-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173852. Licensed CC0.

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