# INTERLEUKIN-1 & REGULATION OF HEMATOPOIESIS

> **NIH NIH R01** · OREGON HEALTH & SCIENCE UNIVERSITY · 1993 · $212,703

## Abstract

The long term objective of this application is to characterize the
molecular mechanisms by which IL-1 enhances the stability and function of
cytokine mRNA in stromal cells of the human hematopoietic microenvironment.
During the past funding period, we have determined that IL-1 governs the
expression of multiple hematopoietic growth factor genes in endothelial
cells and fibroblasts, in large part, by stabilizing gene transcripts.
Specifically, we have found that GM-CSF, G-CSF, IL-1beta, and IL-6 mRNAs
are stabilized in IL-1 stimulated diploid fibroblasts and human vascular
endothelial cells.  Using a cell-free method that faithfully reproduces the
effect of IL-1 on mRNA stability, we have noted that an IL-1-induced RNase
inhibitory factor can be reconstituted by reaction mixtures containing
polysome-bound proteins and cytosolic co-factors.  Using these same
mixtures, we have also demonstrated that IL-1 induces mRNA binding proteins
to bind to specific regions of the GM-CSF mRNA molecule known to
destabilize the transcript.  These methods, combined with promoter/reporter
analysis in intact cells provide us with the tools necessary to achieve our
specific aims; (1) to purify and characterize the biological activity of
cellular factors activated by IL-1 that stabilize cytokine mRNA and (2) to
identify specific attributes (sequences and/or secondary structural
features) of cytokine mRNA chains that permit them to be stabilized in
response to IL-1.

## Key facts

- **NIH application ID:** 3173856
- **Project number:** 5R01CA036306-10
- **Recipient organization:** OREGON HEALTH & SCIENCE UNIVERSITY
- **SAM.gov UEI:** NPSNT86JKN51
- **Principal Investigator:** Grover Carlton Bagby
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $212,703
- **Award type:** 5
- **Project period:** 1984-01-01 → 1995-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173856

## Citation

> US National Institutes of Health, RePORTER application 3173856, INTERLEUKIN-1 & REGULATION OF HEMATOPOIESIS (5R01CA036306-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173856. Licensed CC0.

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