# TRANSFORMING GENES IN INHALATION CARCINOGENESIS

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1987 · $153,592

## Abstract

This application seeks to continue the progress made in analysis of
oncogenes activated in a well-defined experimental tumor model
system.  The novel oncogenes that were previously identified as
being activated in rat nasal squamous carcinomas induced by
inhalation of methylmethane sulfonate, dimethylcarbamyl
chloride, and beta-propiolactone will be further characterized
after molecular cloning of the genes.  Alternative cloning
strategies will include ligation or cotransfection of marker
sequences such as genes for amber mutation suppression or
antibiotic resistance.  Once the novel nasal tumor oncogenes have
been cloned, they will be characterized by DNA sequence analysis
and their chromosomal location, levels of expression in various
tissues and mechanism(s) of activation will be investigated.

The importance of tissue as well as carcinogen specificity in
oncogene activation, and the issue of oncogene activation as a
function of neoplastic progression in an animal tumor model will
be addressed.  Chemical carcinogens known to induce malignant
and benign tumors of several histologic types in the nasal cavity
after exposure by inhalation will be used to generate panels of
tumors.  Activated oncogenes in these tumors will be identified by
the NIH3T3 transfection or the cotransfection/nude mouse
tumorigenesis assays, followed by Southern blot hybridization
analysis.  The hypothesis that certain oncogenes are actively
involved in cellular transformation of particular tissue types and
not others will be tested by transfecting purified oncogenes into
cultures of epithelial cells.

Increased understanding of the molecular mechanisms by which
certain genes are activated in specific cell types during
progression to malignancy and the relationship between
carcinogenic etiology and such oncogene will contribute to
knowledge of the nature of environmental carcinogenesis in
animals and man, and will aid in the development of strategies for
prevention, intervention or protection against cancer for exposed
or susceptible populations.

## Key facts

- **NIH application ID:** 3173889
- **Project number:** 2R01CA036342-04
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** SEYMOUR J GARTE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $153,592
- **Award type:** 2
- **Project period:** 1984-08-01 → 1992-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173889

## Citation

> US National Institutes of Health, RePORTER application 3173889, TRANSFORMING GENES IN INHALATION CARCINOGENESIS (2R01CA036342-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173889. Licensed CC0.

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