# DEVELOPMENT OF PRE-B CELLS

> **NIH NIH R01** · BRANDEIS UNIVERSITY · 1989 · $136,813

## Abstract

The development of immune responses involves, in part, the maturation of a
diverse set of B-cells that can produce antibodies against a multitude of
foreign antigens.  These B-cells arise from stem cells in the bone marrow
and pass through a crucial developmental stage, termed the pre-B cell
stage, during which each B-cell becomes committed to recognizing a
particular antigenic structure.  This commitment involves the recombination
of the heavy and light chain genes that encode the specific antibody
molecule eventually produced by each individual B-cell.  The pre-B cell
differentiation stage is actually a series of stages, each of which is
marked by the recombination of heavy chain, kappa light chain or lambda
light chain antibody genes.  The regulation of pre-B cell maturation will
be studied using a series of Abelson murine leukemia virus transformed cell
lines (Abelson lines) that represent various pre-B differentiation stages.
The mechanisms of regulation and the molecules involved in regulatory
control will be investigated by RNA transcriptional analyses of genes that
may be involved in the regulation of development, DNAase I studies on the
effect of chromosome structure on Ig gene recombination, gene transfer
studies on the effect of antibody proteins on the gene recombination
process and mRNA sequencing studies on the accuracy of gene recombination
in the Lambda light chain gene system.

## Key facts

- **NIH application ID:** 3173937
- **Project number:** 5R01CA036370-06
- **Recipient organization:** BRANDEIS UNIVERSITY
- **SAM.gov UEI:** MXLZGAMFEKN5
- **Principal Investigator:** ERIK SELSING
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $136,813
- **Award type:** 5
- **Project period:** 1983-12-01 → 1990-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173937

## Citation

> US National Institutes of Health, RePORTER application 3173937, DEVELOPMENT OF PRE-B CELLS (5R01CA036370-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173937. Licensed CC0.

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