# GENE TRANSFER AND EXPRESSION USING RETROVIRUS

> **NIH NIH R01** · STRATAGENE CLONING SYSTEMS · 1987 · $107,008

## Abstract

There are three main projects.  1) The construction and study of several
recombinant retroviruses that should permit us to learn more about promoter
interference and trans-acting promoter repression in embryonic cells.  2)
The study of protein/viral DNA interactions in embryonic cells.  The
emphasis is to determine what proteins are involved in viral promoter
"repression" in embryonic cells and to determine their mechanism of
action.  These experiments will utilize DNase hypersensitivity,
footprinting, and Exo III mapping techniques.  3) The microinjection of in
vitro synthesized viral RNA in cultured cells and embryos.  The thrust is
to learn more about the viral replication cycle and to develop a gene
delivery system that will permit efficient insertion of genes, that lack
viral promoter, enhancer, and repressor binding sequences into transgenic
mice in single copy.  An effort will be made to refine the system to
eliminate the need for viral packaging and reinfection.  Finally, in vitro
synthesized RNA microinjection will be used in an attempt to localize the
viral sequences responsible for splicing regulation in retroviruses.

The experiments are significant because they are likely to uncover a potent
trans-acting repressor in mammalian cells.  This repressor may have a more
fundamental role than the inhibition of retroviruses, which will enlarge
our understanding of transcriptional control.  The experiments plan to
localize the viral sequences responsible for promoter inactivation.  This
information should be relevant to those using retroviruses for gene
transfer into stem cells for gene therapy.  Lastly, the understanding of a
mechanism responsible for retroviral inactivation may prove useful in the
designing of future therapies for HTLV mediated diseases.

## Key facts

- **NIH application ID:** 3174034
- **Project number:** 7R01CA036448-05
- **Recipient organization:** STRATAGENE CLONING SYSTEMS
- **Principal Investigator:** JOSEPH A SORGE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $107,008
- **Award type:** 7
- **Project period:** 1987-07-01 → 1991-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174034

## Citation

> US National Institutes of Health, RePORTER application 3174034, GENE TRANSFER AND EXPRESSION USING RETROVIRUS (7R01CA036448-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174034. Licensed CC0.

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