# ACTIVATION OF ONCOGENES IN RADIATION CARCINOGENESIS

> **NIH NIH R01** · UNIVERSITY OF IOWA · 1988 · $117,712

## Abstract

The overall goal of this proposal is to investigate the activation of
oncogenes in radiation carcinogenesis.  Recently, our laboratory has
identified an activated k-ras oncogene in x-ray transformed 10T 1/2 cells
in culture.  Evidence suggests that the k-ras oncogene has been activated
by somatic mutation.  We are proposing to extend these results to
investigate whether other types of radiation induced-transformation
activate the same oncogene, and by the same mechanism of activation, in 10T
1/2 clones transformed by UV-light, fission-spectrum neutrons and x-rays.
Our second aim is to extend the results of k-ras activation in x-ray
transformed cells.  We will identify oncogenic activation in a
x-irradiation induced fibrosarcoma in C3H mouse and compare these results
to the activated k-ras in x-ray transformed C3H cells (10T 1/2) in
culture.  These results will suggest whether x-ray transformation activates
the same oncogene (and mechanism of activation) in vitro and in vivo.
Additionally, we are proposing to investigate oncogenic activation in x-ray
induced immortalization of primary rat embryo cells in culture associated
with, and without, tumorigenicity.  These studies will more clearly define
the earlier events (immortalization) of radiation induced tumorigenesis.
The third aim of this proposal is to investigate the timing of k-ras
oncogene activation after x-irradiation of 10T 1/2 cells.

The research in this proposal will be accomplished using the techniques of
1) hybridization for DNA and RNA analysis, 2) DNA transfection experiments,
3) molecular cloning by construction of genomic libraries, and 4) DNA
sequencing.  The results will identify activated oncogenes and define the
molecular mechanisms of activation in the multistep events of radiation
carcinogenesis.  The knowledge gained from these investigations may
eventually lead to methods of treatment and prevention of radiation induced
neoplasia in man.

## Key facts

- **NIH application ID:** 3174073
- **Project number:** 5R01CA036483-05
- **Recipient organization:** UNIVERSITY OF IOWA
- **SAM.gov UEI:** Z1H9VJS8NG16
- **Principal Investigator:** DUANE L GUERNSEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $117,712
- **Award type:** 5
- **Project period:** 1983-12-01 → 1988-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174073

## Citation

> US National Institutes of Health, RePORTER application 3174073, ACTIVATION OF ONCOGENES IN RADIATION CARCINOGENESIS (5R01CA036483-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174073. Licensed CC0.

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