# ISOLATION AND CLONING OF HUMAN DNA REPAIR GENES

> **NIH NIH R01** · UNIVERSITY OF TX MD ANDERSON CAN CTR · 1987 · $67,203

## Abstract

An assay has been developed in which the DNA repair deficiency of the human
genetic disease Xeroderma Pigmentosum is transiently complemented by the
introduction by microinjection of cytoplasmic poly(A)+RNA derived from Hela
cells.  This assay will be utilized to isolate a cDNA copy of repair mRNAs
by zoom cloning techniques.  The cDNA copy will be used as a probe to
identify a clone containing the genomic copy of the repair gene from a
human, recombinant DNA library.  Structural analyses of the cloned genes
and biochemical characterization of their expression products will be
performed.  These studies should help to elucidate the molecular mechanisms
of human, DNA excision repair and the pathobiology of XP.

## Key facts

- **NIH application ID:** 3174087
- **Project number:** 5R01CA036486-04
- **Recipient organization:** UNIVERSITY OF TX MD ANDERSON CAN CTR
- **SAM.gov UEI:** S3GMKS8ELA16
- **Principal Investigator:** RANDY J LEGERSKI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $67,203
- **Award type:** 5
- **Project period:** 1984-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174087

## Citation

> US National Institutes of Health, RePORTER application 3174087, ISOLATION AND CLONING OF HUMAN DNA REPAIR GENES (5R01CA036486-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174087. Licensed CC0.

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