# IMMUNOGLOBULIN GENE EXPRESSION IN MYELOMA MUTANTS

> **NIH NIH R01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 1990 · $155,024

## Abstract

The long term goals of this research are to understand the
regulation of immunoglobulin heavy chain mRNA processing and
expression during normal and neoplastic B cell development.
Mouse myeloma tumor cells and lymphoma cells are being used,
respectively, as models of plasma cells and earlier B cell stages
and memory cells.  Differential production of membrane and
secretory encoding mRNAs from a single IgG gene has been shown
to be regulated by post-transcriptional processing events,
differential polyadenylation and/or splicing.  We propose to study
the mechanism of this regulation by making altered gene
constructs and transfecting them back into the myeloma and
lymphoma cells to test the role of various cis-acting sequences.
Areas of DNA near the membrane encoding poly A sites will be
sequenced and compared with the already sequenced secretory
poly A sites.  In vitro polyadenylation systems from myeloma and
lymphoma cells will be established.  Requirements for cis-acting
sequences and putative trans-acting, stage-specific factors will be
investigated in the vitro system.  The role of transcription
termination sites in regulated membrane and secretory mRNA
production will be investigated by transcription run-on assays,
analysis of nulcease resistant species, DNA sequencing and tests
for biological functionality.

Differences in transcription rate alone cannot account for the
tremendous differences observed in immunoglobulin mRNA
abundancies during B cell development.  We propose to measure
immunoglobulin heavy chain mRNA turnover in cells representing
different B cell stages and correlate these with abundancy,
transcription rate and nuclear transport and stability.

## Key facts

- **NIH application ID:** 3174216
- **Project number:** 5R01CA036606-08
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **SAM.gov UEI:** MKAGLD59JRL1
- **Principal Investigator:** Christine Ann Milcarek
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $155,024
- **Award type:** 5
- **Project period:** 1983-08-01 → 1993-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174216

## Citation

> US National Institutes of Health, RePORTER application 3174216, IMMUNOGLOBULIN GENE EXPRESSION IN MYELOMA MUTANTS (5R01CA036606-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174216. Licensed CC0.

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