# CELLULAR AND MOLECULAR EVENTS IN B LYMPHOCYTE ACTIVATION

> **NIH NIH R01** · DUKE UNIVERSITY · 1989 · $200,850

## Abstract

The stimulation of B lymphocytes culminates in the differentiation of these
cells into high rate antibody secreting cells.  Outside of the well
characterized alterations in the expression of immunoglobulin and related
genes and of several cell surface molecules, little is known about the
genetic events which are associated with differentiation to antibody
secretion.  In this application, we will identify and characterize genes
whose expression is regulated during the late stages of B lymphocyte
differentiation.  The specific aims are 1) to characterize the kinetics and
specificity of expression of endogenous mouse mammary tumor virus (MMTV)
genes during differentiation.  The expression of one of these genes, Mtv-
9, is associated with B cell but not T cell differentiation; 2) to identify
the mechanisms responsible for increased steady state levels of env
transcripts during differentiation, and to identify the cis regulatory
elements that are required for Mtv-9 transcription in B lymphocytes; the
regions which confer tissue specificity will be determined, and the
sequences recognized by DNA binding proteins will be identified; 3) to
determine whether negative regulation contributes to the developmental
expression or tissue specificity of Mtv-9, and to identify the cis
regulatory sequences and trans-acting factors that are involved in the
negative regulation; 4) to isolate other genes whose expression is
regulated during the late phases of B cell differentiation, and to
characterize the tissue distribution and ontogeny of expression of these
genes.  A preliminary analysis of the regulation of expression of these
genes will be performed.  Results of these experiments will contribute to
our understanding of the B cell differentiative process and should provide
novel information concerning virus-lymphocyte interactions.  Moreover,
these studies may suggest methods for effective intervention in the
treatment of B cell malignancies, either by interfering with replication
or by modulating the differentiative process.

## Key facts

- **NIH application ID:** 3174240
- **Project number:** 2R01CA036642-09
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** RONALD B CORLEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $200,850
- **Award type:** 2
- **Project period:** 1987-07-01 → 1992-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174240

## Citation

> US National Institutes of Health, RePORTER application 3174240, CELLULAR AND MOLECULAR EVENTS IN B LYMPHOCYTE ACTIVATION (2R01CA036642-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174240. Licensed CC0.

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