# DNA REPAIR AFTER POLYCYCLIC HYDROCARBON ADMINISTRATION

> **NIH NIH R01** · DARTMOUTH COLLEGE · 1989 · $154,271

## Abstract

06-Methylguanine DNA methyltransferase (OMMT) is an important
protein that functions in the repair of 06-methylguanine lesions
in DNA that may be of some importance in carcinogenesis, as well
as in cancer chemotherapy.  The long-term objective of the research
is to understand the nature of the OMMT gene in mammalian systems,
the details of its molecular biology, and the manner of its
regulation.  The specific aims of this proposal are to:  a) isolate
the human OMMT gene from 2 cosmids (102 and A-36) that upon
transfection are able to confer resistance to
chloroethylnitrosourea (CNU) in CHO-D422 (mex-) cells, determine
the DNA sequence of this gene, and examine its conservation in the
human, rat, mouse and hamster; b) characterize the human OMMT mRNA,
determine its rate of transcription in the livers of gamma-
irradiated rats by using northern hybridization analysis, and
ascertain if methylated OMMT can act as a trans-activator:  c)
determine the amino acid sequence of OMMT from protein that has
been separated by SOS-PAGE, prepare antibodies to OMMT by using
synthesized oligopeptides, and ascertain the stability of OMMT in
the livers of the irradiated rats; and d) determine the specificity
of OMMT for ras sequences that have been methylated at various
guanines within and in the region of codon 12 by using synthetic
15 mer double-stranded oligodeoxyribonucleotides; and e)
characterize the DNA repair capabilities of Cosmid 102-transformed
excision repair-deficient CHO mutant cells, uV 5, UV 20 and UV 41,
in regard to nitrosoureas, MNNG, and cis-platinum.

## Key facts

- **NIH application ID:** 3174249
- **Project number:** 2R01CA036679-07
- **Recipient organization:** DARTMOUTH COLLEGE
- **SAM.gov UEI:** EB8ASJBCFER9
- **Principal Investigator:** EDWARD BRESNICK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $154,271
- **Award type:** 2
- **Project period:** 1983-05-01 → 1992-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174249

## Citation

> US National Institutes of Health, RePORTER application 3174249, DNA REPAIR AFTER POLYCYCLIC HYDROCARBON ADMINISTRATION (2R01CA036679-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174249. Licensed CC0.

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