# DNA REPAIR AFTER POLYCYCLIC HYDROCARBON ADMINISTRATION

> **NIH NIH R01** · UNIVERSITY OF NEBRASKA MEDICAL CENTER · 1988 · $124,240

## Abstract

A major hypothesis in chemical carcinogenesis is that an interaction
between the chemical and cellular is a prerequisite for transformation.
Cells generally possess DNA repair pathways for at least some of the
resulting damage.  The absence or deficiency of a repair process may
predispose to cancer.  This research will investigate the repair of two
types of DNA damage, that induced by either benzo(a)-pyrene or
methylnitrosourea; in the latter case, repair of a specific lesion,
0/6-methylguanine.  Previous experiments with hamster tracheal epithelial
cells suggest that benzo(a)pyrene adducts in DNA are repaired by a pathway
that differs from ultraviolet light-induced pyrimidine dimers.  This will
be confirmed by purification of the enzymes involved.  Furthermore, DNA
repair-deficient cells will be isolated and used both for comparison of the
different repair mechanisms, as well as for experiments to isolate the
repair genes.  With regard to repair of 0/6-methylguanine, we have already
transfected DNA from human liver into repair-deficient Chinese hamster
ovary cells and selected a clone that expresses the methyltransferase
gene.  The gene will now be isolated from these cells and used to obtain
information on the mRNA and protein structure.  Antibodies to the protein,
will be obtained using, as immunogen, either the purified protein or a
synthetic polypeptide, the sequence of which can be obtained directly from
the DNA sequence.  The antibodies and genetic probes will be used to assess
variations within the human population.

## Key facts

- **NIH application ID:** 3174254
- **Project number:** 5R01CA036679-06
- **Recipient organization:** UNIVERSITY OF NEBRASKA MEDICAL CENTER
- **SAM.gov UEI:** G15AG3BLLMH4
- **Principal Investigator:** EDWARD BRESNICK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $124,240
- **Award type:** 5
- **Project period:** 1983-05-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174254

## Citation

> US National Institutes of Health, RePORTER application 3174254, DNA REPAIR AFTER POLYCYCLIC HYDROCARBON ADMINISTRATION (5R01CA036679-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174254. Licensed CC0.

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