# STRUCTURAL STUDIES OF THE PRODUCTS OF THE H-2 COMPLEX

> **NIH NIH R01** · NATIONAL JEWISH HEALTH · 1987 · $133,906

## Abstract

This work covers several areas of study of the structure of the murine Ia
antigens, which are integral membrane glycoproteins encoded by genes in the
I-region of the H-2 complex (MHC) of the mouse.  The first objective is the
elucidation of the structures of the oligosaccharides carried by Ia
antigens.  We will complete our studies of the carbohydrate moieties of the
alpha and beta chains of the I-Ak antigen using radiochemical
techniques.  The second objective is the localization of the antigenic
determinants of the I-Ak antigen.  Monoclonal antibodies will be used to
define the relationship between the antigenic structure and the primary
structure of the I-Ak antigen, since a large number of reagents are
available for this antigen.  If possible, this approach will be extended to
the I-Ad antigen.  The third objective is to carry out a detailed
examination of the orientation of the Ia antigens in the lipid bilayer of
the plasma membrane, including the development of markers for the
extracellular, cytoplasmic, and membrane-integrating portions of the
molecules.  Within this general objective are experiments designed to
search for potential interactions of Ia antigens with other membrane
proteins or with cytoskeletal elements. A corollary of these experiments
will be a search for potential interactions between Ia molecules, the
foreign antigen, and the antigen-specific receptor of T cells.

Experiments to accomplish the three objectives fall within the areas of
immunochemistry and biochemistry.  Ia antigens will be purified by affinity
chromatography on monoclonal antibody columns, chains will be separated by
SDS-PAGE or by ion exchange chromatography in acid-urea, and antigenic
fragments will be monitored by indirect immunoprecipitation or by RIA.  The
primary approach to be employed in documenting the interaction of Ia
antigens with other molecules will be the use of chemical cross-linkers.
(CS)

## Key facts

- **NIH application ID:** 3174274
- **Project number:** 5R01CA036700-05
- **Recipient organization:** NATIONAL JEWISH HEALTH
- **SAM.gov UEI:** VLQKFEEJ3NE9
- **Principal Investigator:** JOHN H FREED
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $133,906
- **Award type:** 5
- **Project period:** 1983-07-01 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174274

## Citation

> US National Institutes of Health, RePORTER application 3174274, STRUCTURAL STUDIES OF THE PRODUCTS OF THE H-2 COMPLEX (5R01CA036700-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174274. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*