# EFFECTS OF FLUOROPYRIMIDINE ON RNA STRUCTURE & FUNCTION

> **NIH NIH R01** · YALE UNIVERSITY · 1987 · $45,380

## Abstract

We have evaluated the alterations induced in RNA by 5-fluorouracil.  We
have specifically identified changes in mRNA and rRNA processing.  Our
results indicate that a likely site of 5-fluorouracil action is from
incorporation in SnRNA.  These small molecular weight uracil rich RNA's are
required for various steps in the processing of the larger RNA which are
the precursors to cytoplasmic mRNA and rRNA.  We plan to continue work in
this area of RNA metabolism.  Two base anologues will be used,
6-Thioguanine and 5-fluorouracil, a purine and a pyrimidine.  We have
established the methods for evaluating mRNA for dihydrofolate reductase and
cMyc.  Specific probes for the mRNA transcript and certain nitrons will be
used to more clearly delineate the process of mRNA formation to the mature
form.  In addition, the effects of the various SnRNA's on these nuclear RNA
processing steps will be evaluated.  The mechanism by which the mature RNA
is transported to the cytoplasm will be studied also.  This is an
extragenomic site of gene control which we have findings which are
suggestive of a site for drug action.  Although we will use clinically
available base analogues, the results will provide a better understanding
of our knowledge in this unique area of RNA metabolism.

## Key facts

- **NIH application ID:** 3174295
- **Project number:** 7R01CA036704-05
- **Recipient organization:** YALE UNIVERSITY
- **SAM.gov UEI:** FL6GV84CKN57
- **Principal Investigator:** EDWIN C CADMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $45,380
- **Award type:** 7
- **Project period:** 1987-09-01 → 1990-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174295

## Citation

> US National Institutes of Health, RePORTER application 3174295, EFFECTS OF FLUOROPYRIMIDINE ON RNA STRUCTURE & FUNCTION (7R01CA036704-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174295. Licensed CC0.

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