# PHOSPHOLIPID-CA2+ AND PHOSPHOPROTEINS IN LEUKEMIC CELLS

> **NIH NIH R01** · EMORY UNIVERSITY · 1987 · $133,168

## Abstract

The specific aims of this research cover four major areas of the
newly-identified phospholipid/Ca2+-stimulated protein phosphorylation
system in human leukemic cells.  (1)\Phospholipid-sensitive
Ca2+-dependent protein kinase will be purified to homogeneity from
leukemic cells from patients, and its properties and mechanism of
activation by phospholipid and Ca2+ will be studied.  Immunocytochemistry
of the enzyme in normal neutrophils, leukemic cells from patients, and HL60
and K562 cells in culture will be studied using polyclonal antibodies to
the enzyme already developed in this lab.  (2)\Endogenous substrate
proteins for the enzyme will be searched for, identified, and
characterized.  Attention will be paid to the variation in the substrates
so that their pathophysiologic relevance can be suggested.  Monoclonal
antibodies to the substrates "specific" to leukemic cells will be
developed; and their ability to inhibit certain leukocyte functions, as
well as their diagnostic and therapeutic usefulness in leukemias, will be
explored.  Endogenous protein phosphorylation stimulated by
calmodulin/Ca2+, cAMP, and cGMP also will be carried out so that the
interplays among the cellular mediators at the level of protein
phosphorylation in the biology and pathology of human leukocytes can be
studied.  (3)\Alterations in the enzyme (activity level and subcellular
distribution) and substrates will be investigated in HL-60 stimulated to
terminally differentiate by dimethylsulfoxide and phorbol ester.
(4)\Structure-activity relationship of alkyl-lysophospholipid analogs to
inhibit the enzyme system will be examined, and the results will be
compared with their clinical efficacy (if known) and their ability to
affect leukemic cell growth in culture.  (B)

## Key facts

- **NIH application ID:** 3174348
- **Project number:** 5R01CA036777-04
- **Recipient organization:** EMORY UNIVERSITY
- **SAM.gov UEI:** S352L5PJLMP8
- **Principal Investigator:** JYH-FA KUO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $133,168
- **Award type:** 5
- **Project period:** 1984-03-01 → 1988-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174348

## Citation

> US National Institutes of Health, RePORTER application 3174348, PHOSPHOLIPID-CA2+ AND PHOSPHOPROTEINS IN LEUKEMIC CELLS (5R01CA036777-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174348. Licensed CC0.

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