# PROTEIN KINASE C IN CELL GROWTH AND DIFFERENTIATION

> **NIH NIH R01** · EMORY UNIVERSITY · 1989 · $157,004

## Abstract

The overall goals of this renewal application cover primarily the
molecular, immunological, pharmacological, regulatory and
functional aspects of protein kinase C (PKC) in cell growth and
differentiation.  The activity level, immunoreactivity and
immunocytochemical localization (both at the light and electron
microscopic level of PKC, using anit-PKC antisera developed in
the PI's lab, and phosphorylation of endogenous substrate proteins
for PKC in HL60, K562, KG-1, KG-1a, CHO and E7SKS cells will
be examined, so that the role of the PKC system in the TPA-
induced cell differentiation can be assessed.  Similar studies will
be conducted using BHK-21 and its temperature sensitive mutants
(blocked at different phases of cell cycle), and BALB/c-3T3 and
its transformed sublines (M-MSV, K-BALB and 3T12-3), so that
the role of the PKC system in cell cycle progression and cell
growth can be further investigated.  In addition, the effects of
existing anti-cancer agents and synthetic analogs of
alkyllsophospholipid and phosphatidylcholine on PKC activity and
directional PKC translocaton, protein phosphorylation and cell
differentiation induced by TPA will be examined, so that PKC can
be established as a potential site of actions of these agents.  It is
hoped that the proposed research would yield new knowledge
regarding the regulation of cell growth and differentiation, which
would potentially aid cancer prevention and therapy.

## Key facts

- **NIH application ID:** 3174349
- **Project number:** 5R01CA036777-06
- **Recipient organization:** EMORY UNIVERSITY
- **SAM.gov UEI:** S352L5PJLMP8
- **Principal Investigator:** JYH-FA KUO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $157,004
- **Award type:** 5
- **Project period:** 1984-03-01 → 1993-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174349

## Citation

> US National Institutes of Health, RePORTER application 3174349, PROTEIN KINASE C IN CELL GROWTH AND DIFFERENTIATION (5R01CA036777-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174349. Licensed CC0.

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