# RADIATION AND DRUG EFFECTS ON TUMOR CELL

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1991 · $182,283

## Abstract

The overall objective of this research program is to determine the
importance of various tumor cell subpopulations in predicting a tumor's
response to single and combined modality therapies.  Cell subpopulations
will be derived from solid KHT, RIF-1 or EMT6/Ro tumors using centrifugal
elutriation.  Experiments are proposed to determine the proportion and cell
cycle location of quiescent (Q) cells and to measure growth imbalance in
these solid tumor models using acridine orange (AO) staining and flow
cytometric (FCM) analysis.  The location, with respect to cell cycle phase
and proliferation state, of oxygen-deficient (hypoxic) tumor cells will be
characterized using radiation cell survival curve analysis, combinations of
centrifugal elutriation plus viable cell sorting (Hoechst 33342) and the
identification of hypoxic cells through the use of labelled hypoxic cell
sensitizers.  In situ sensitivity differences of various tumor cell
subpopulations (including hypoxic and quiescent cells) will also be
determined.  It is further an objective to assess which subpopulations are
most affected by radiation, sensitizers and selected chemotherapeutic
agents.  Possible interactions of sensitizers, drugs and radiation in terms
of their effects on the total tumor as well as specific subpopulations will
be evaluated.  These studies should allow the development of mechanisms of
interactions for the different combined modality treatments.  Experiments
aimed at determining the response of tumors and their subpopulations to
different fractionated dose regimen also will be performed.  In particular
the effects of fractionated therapies on cell cycle redistribution and
quiescent cell recruitment will be measured.  These investigations should
produce information which will improve the understanding of the role played
by various cell subpopulations in determining the overall response of
tumors to therapy and which may guide the efforts for the most effective
use of combinations of radiation, chemotherapeutic agents and sensitizers.

## Key facts

- **NIH application ID:** 3174441
- **Project number:** 5R01CA036858-08
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** DIETMAR W SIEMANN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $182,283
- **Award type:** 5
- **Project period:** 1984-05-01 → 1993-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174441

## Citation

> US National Institutes of Health, RePORTER application 3174441, RADIATION AND DRUG EFFECTS ON TUMOR CELL (5R01CA036858-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174441. Licensed CC0.

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