# MACROMOLECULAR TRANSPORT IN NEOPLASTIC CAPILLARY BEDS

> **NIH NIH R01** · MASSACHUSETTS GENERAL HOSPITAL · 1993 · $285,638

## Abstract

In its journey from the blood stream to cancer cells, a therapeutic agent
encounters two barriers: the microvascular wall and the interstitium.
The overall goal of our research is to develop a quantitative
understanding of these two barriers to transport in tumors, and to
develop novel strategies to overcome these barriers for improved cancer
detection and treatment.  More specifically, the aims for this renewal
project are (1) to characterize binding kinetics in vivo between
monoclonal antibodies and tumor-associated antigens and (2) to optimize
two-step approaches for antibody-mediated delivery.

Transport of fluorescently labelled molecules of interest will be
measured in normal tissue and human tumors grown in the dorsal chamber in
immunodeficient mice using two techniques developed in our laboratory:
dual-fluorescence intravital microscopy (DFIM) and fluorescence recovery
after photobleaching (FRAP).  We will analyze these data using
appropriate mathematical models.  These models will also be used to
develop guidelines for optimal dose and schedule of therapeutic agents.

The proposed investigation is a logical extension of our previous
research on the transport of nonspecific molecules in the rabbit ear
chamber preparation.  This renewal project will provide novel and
-crucial data on the movement of anti-tumor monoclonal antibodies in
human tumor xenografts.  It not only represents a major step towards a
fundamental understanding of transport and binding in tumors, but is also
essential to improve the delivery of novel as well as conventional
anti-tumor agents.

## Key facts

- **NIH application ID:** 3174531
- **Project number:** 2R01CA036902-10
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **SAM.gov UEI:** FLJ7DQKLL226
- **Principal Investigator:** Rakesh K. Jain
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $285,638
- **Award type:** 2
- **Project period:** 1984-01-01 → 1993-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174531

## Citation

> US National Institutes of Health, RePORTER application 3174531, MACROMOLECULAR TRANSPORT IN NEOPLASTIC CAPILLARY BEDS (2R01CA036902-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174531. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*