# IMMUNOBIOLOGY OF HYBRID RESISTANCE

> **NIH NIH R01** · UT SOUTHWESTERN MEDICAL CENTER · 1989 · $101,442

## Abstract

Hybrid and allogeneic resistance to bone marrow allografts in irradiated
mice is an H-2 specific reaction against Hemopoietic histocompatibility
(Hh) antigens.  Natural Killer (NK) cells mediate this rejection
phenomenon, and in doing so may utilize Hh-specific receptors.  This offers
the opportunity to investigate the nature of antigen specific receptors on
NK cells, a cell type extremely important in surveillance against tumor
cells.  The nature of immunoglobulin receptors on B cells and T cell
receptors have been well studied and characterized; if receptors are
discovered on NK cells, analysis of a third-type of lymphocyte receptor may
be possible.  However, one must first determine whether or not marrow graft
rejection is mediated by circulating 'natural antibodies' (Aim 1).  In vivo
and in vitro anti-Hh reactions will be assessed, using marrow graft
rejection, lysis of Hh+ and Hh- tumor cells by purified NK cells and
inhibition of marrow colony-formation in vitro by NK cells, with or without
serum present.  We will secrete for NK cells with specificity for Hh
antigens, using adsorption to monolayers of tumor cells expressing NK
target structures but perhaps no Hh antigens, and vice versa.  NK cells
specific for Hh antigens will be expanded and cloned.  Antibodies to these
receptors will be sought (Aim 2).  T suppressor cells capable of inhibiting
hybrid resistance in a specific fashion have been described.  We will
attempt to detect, characterize, clone and expand such cells and test for
anti-Hh receptors (Aim 3).  Finally, we will assess the role of
interferons, IL-2 and other cytokines in the mechanism of marrow allograft
rejection; the key target cell probably is the NK cell (Aim 4).

## Key facts

- **NIH application ID:** 3174588
- **Project number:** 5R01CA036922-06
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **SAM.gov UEI:** YZJ6DKPM4W63
- **Principal Investigator:** Michael Bennett
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $101,442
- **Award type:** 5
- **Project period:** 1984-03-01 → 1990-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174588

## Citation

> US National Institutes of Health, RePORTER application 3174588, IMMUNOBIOLOGY OF HYBRID RESISTANCE (5R01CA036922-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174588. Licensed CC0.

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