# STRUCTURE-FUNCTION STUDIES OF ALTERED ONCOGENIC PROTEINS

> **NIH NIH R01** · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · 1988 · $35,625

## Abstract

The transformation of cells by the retrovirus, Rous sarcoma virus (RSV), is
attributed to the cellular function of a 60 kilodalton protein product of
the viral src gene, pp60v-src.  This 60 kilodalton protein is a protein
kinase, specifically phosphorylating tyrosine residue.

It is generally appreciated that pp60v-src may have several cellular
targets whose physiological functions are affected by phosphorylation of
one or more tyrosine residues.  These individual enzyme-substrate
interactions could account for the changes in cells as they undergo
transformation, i.e., rounded morphology, anchorage-independent growth,
loss of density-dependent inhibition of division, increased ion and glucose
transport, production of proteases (i.e., plasminogen activator), and loss
of cell surface fibronectin.  Infection with partially defective mutants of
RSV may result in the selective appearance of one or more of these cellular
phenotypes.  For the most part, these partially defective mutants have been
prepared by a random mutagenic procedure.  We now are applying recently
developed procedures that will allow mutagenesis in defined areas of
pp60v-src and ultimately, specific to certain amino acids.  By this
procedure we hope to define the composition of the active site of the
enzyme and the corresponding binding sites to putative individual proteins
that result in the pleiotypic changes in the transformation process.  (X)

## Key facts

- **NIH application ID:** 3174592
- **Project number:** 3R01CA036928-04S1
- **Recipient organization:** MASSACHUSETTS INSTITUTE OF TECHNOLOGY
- **SAM.gov UEI:** E2NYLCDML6V1
- **Principal Investigator:** JOHN M BUCHANAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $35,625
- **Award type:** 3
- **Project period:** 1984-03-15 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174592

## Citation

> US National Institutes of Health, RePORTER application 3174592, STRUCTURE-FUNCTION STUDIES OF ALTERED ONCOGENIC PROTEINS (3R01CA036928-04S1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174592. Licensed CC0.

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