# BIOCHEMICAL RESPONSES OF HYPOXIC CELLS TO CHEMOTHERAPY

> **NIH NIH R01** · GEORGE WASHINGTON UNIVERSITY · 1992 · $133,661

## Abstract

DESCRIPTION:  (Adapted from applicant's abstract) The overall goal of these
studies is to understand how hypoxia and thiol depletion can modulate the
responses of cells to cytotoxic therapy, the repair of misonidazole induced
DNA damage under thiol depletion and supplementation will be correlated
with cytotoxicity.  Additional studies with model alkylating agents, EMS
and L-PAM, will be done to compare the responses of normally aerated cells
with hypoxic and reoxygenated cells.  Experiments will also be performed to
extend assessment of the relative resistance of hypoxic cells and
reoxygenated cells to teniposide To assess cytotoxicity, colony formation
assays will be used.  DNA repair in EMT 6 mouse mammary tumor cells will be
determined using alkaline elution techniques.

## Key facts

- **NIH application ID:** 3174622
- **Project number:** 5R01CA036946-08
- **Recipient organization:** GEORGE WASHINGTON UNIVERSITY
- **SAM.gov UEI:** ECR5E2LU5BL6
- **Principal Investigator:** KATHERINE A. KENNEDY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $133,661
- **Award type:** 5
- **Project period:** 1984-04-01 → 1994-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174622

## Citation

> US National Institutes of Health, RePORTER application 3174622, BIOCHEMICAL RESPONSES OF HYPOXIC CELLS TO CHEMOTHERAPY (5R01CA036946-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174622. Licensed CC0.

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