# STUDY OF THE PX REGION OF HTLV I & II

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1988 · $148,141

## Abstract

The goal of this proposal is to understand the mechanisms of cell
transformation by the human T cell leukemia viruses types I and II
(HIV-I and II).  The focus of research over the past two years has
been in the p42 transactivator protein encoded by the pX region.
The focus of attention in the coming years will be in the newly
described pp27 and pp21 pX region encoded proteins.  The
structure and function of these proteins will be investigated.  The
combined effects of expression of all three proteins, p42, pp27,
and pp21 on viral gene expression, on the expression of cellular
genes, and on T cell transformation will also be investigated.

The objectives of the proposal are:

1. To determine the structure and coding sequences of the pX
encoded pp27 and pp21 protein.  Specific aims include:  a)
development of specific antisera to the separate coding
regions of pp27 and pp21; b) protein sequence analysis of the
pp27 and pp21 proteins; c) characterization of the pp27 and
pp21 messenger RNA species; d) large scale production and
purification of the p42, pp27 and pp21 proteins.
2. To determine the role of the p42, pp27, and pp21 proteins in
the regulation of viral gene expression.  Specific aims
include:  a) construction of vectors that express the pp27
and pp21 proteins in transient transfection assays; b)
construction of cell lines that express stably combinations
of the p42, pp27, and pp21 proteins; c) analysis of the
effects of these proteins on HIV-I and HIV-II LTR directed
gene expression; d) fine mapping of the cis-acting regions
within the LTR and viral genes responsive to the action of
these proteins.
3. To determine the role of the p42, pp27, and pp21 proteins in
regulation of cellular genes.  Specific aims include:  a)
analysis of the altered gene expression in T and B cell lines
that constitutively produce the pX encoded proteins singly
or in combination.
4. To determine if primary lymphocytes can be transformed by
pX region products.  Specific aims include construction of
new vectors suitable for primary cell transformation.

It is expected that this work outlined in this proposal will take
five years to complete.

## Key facts

- **NIH application ID:** 3174641
- **Project number:** 5R01CA036974-05
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** WILLIAM A. HASELTINE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $148,141
- **Award type:** 5
- **Project period:** 1984-08-01 → 1992-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174641

## Citation

> US National Institutes of Health, RePORTER application 3174641, STUDY OF THE PX REGION OF HTLV I & II (5R01CA036974-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174641. Licensed CC0.

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