# MULTIDRIG RESISTANCE AND P-GLYCOPROTEIN

> **NIH NIH R01** · ONTARIO CANCER INSTITUTE · 1987 · $116,072

## Abstract

The objective of this application is to investigate what roles mutations
resulting in multidrug resistance play in human cancer.  Such mutations are
thought to limit successful chemotherapeutic treatment of advanced
disease.  The immediate aim is to develop sensitive and specific probes for
detecting multidrug resistance cells in patient biopsies.  This will be
accomplished by generating monoclonal antibodies against the 170,000 dalton
cell surface P-glucoprotein shown to be expressed in multidrug resistance
cells.  Heteroantisera against this protein already developed will be used
to screen biopsy samples from cancer patients using the highly sensitive
immunoblot (Western blot) procedure.  DNA sequence specific for human
P-glycoprotein will be cloned and used as probes for multidrug resistance
mutations.  Multidrug-resistant cell will be isolated in vitro from cell
lines established from patient tumors.  These will be studied and compared
with resistant cells found in patients.

This program should provide significant information concerning how
drug-resistance limits chemotherapeutic treatment of malignancies.  It may
in addition, provide new vistas for improving therapeutic management.

## Key facts

- **NIH application ID:** 3174826
- **Project number:** 2R01CA037130-04
- **Recipient organization:** ONTARIO CANCER INSTITUTE
- **Principal Investigator:** VICTOR LING
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $116,072
- **Award type:** 2
- **Project period:** 1984-04-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174826

## Citation

> US National Institutes of Health, RePORTER application 3174826, MULTIDRIG RESISTANCE AND P-GLYCOPROTEIN (2R01CA037130-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174826. Licensed CC0.

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