# SV40-INDUCED CHANGES IN GROWTH REGULATION IN HOST CELLS

> **NIH NIH R01** · MICHIGAN STATE UNIVERSITY · 1988 · $132,750

## Abstract

The objective of this research is to understand the mechanism(s)
by which S-phase specific genes are regulated in normal and SV40-
infected cells.  Since the expression of these genes is correlated
with the growth state of the cell, it is hoped that an understand of
their regulation will contribute to an understanding of the control
of cell growth in normal and virus-infected cells.  This
understanding will in turn be important to the study of oncogenic
transformation, since one of the properties of transformed cells is
that they fail to regulate their growth in a normal way.

The approach taken to investigating the regulation of S-phase
specific genes will be a molecular one, using the thymidine kinase
(TK) gene as a model system.  TK expression is regulated during
the cell cycle, being low in G0 and G1 phase cells, and increasing
as the cells enter S phase.  Both genomic and cDNA TK clones
will be used to construct a series of mutant and hybrid TK genes.
These altered genes will then be transfected into tissue culture
cells, and the regulation of their expression will be examined.
Using these techniques, the following questions concerning TK
regulation will be addressed:  1) At what levels does regulation of
the gene occur (transcriptional/post-transcriptional, etc.)?  2)
What TK DNA sequences are responsible for this regulation?  3)
What is the role of viral proteins in inducing the TK gene?  4)
What cellular factor or factors are involved in inducing the TK
gene?

## Key facts

- **NIH application ID:** 3174873
- **Project number:** 5R01CA037144-05
- **Recipient organization:** MICHIGAN STATE UNIVERSITY
- **SAM.gov UEI:** R28EKN92ZTZ9
- **Principal Investigator:** SUSAN E CONRAD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $132,750
- **Award type:** 5
- **Project period:** 1984-03-01 → 1990-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174873

## Citation

> US National Institutes of Health, RePORTER application 3174873, SV40-INDUCED CHANGES IN GROWTH REGULATION IN HOST CELLS (5R01CA037144-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174873. Licensed CC0.

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