# MONOCLONAL ANTIBODIES IN THE IMMUNOHISTOCHEMICAL CLASS

> **NIH NIH R01** · CITY OF HOPE NATIONAL MEDICAL CENTER · 1987 · $149,043

## Abstract

The purpose of this research project is to screen, and
comprehensively test monoclonal antibodies (Mabs) by
immunohistochemistry, in order to identify those capable of
increasing the accuracy of histological diagnosis of, alternatively,
those which recognize marker substances of prognostic
significance.  Mabs obtained from various external sources as well
as produced in our own laboratory will be extensively screened
against a panel of normal human tissues and selected neoplasms of
variable histogenesis and degrees of differentiation.  Frozen
tumors from our frozen tumor bank will serve as the "golden rule"
for comparative studies with paraffin embedded tissues.  Freeze-
dried (unfixed) tissue, ethanol-fixed and formalin-fixed tissues, all
embedded in paraffin will be tested with each Mab.  Various
immunohistochemical methods will be initially employed on
frozen and paraffin embedded tissues to maximize selection of
the optimal handling for each antigen/antibody.  Antibodies which
after rigorous screening show tissue or tumor discriminatory
capacity will be tested against a larger group of well
characterized neoplasms of appropriate type depending on the
specificity shown during screening.  To facilitate screening in
search of markers of biological significance, especially prepared
multi-tumor blocks containing samples of neoplasms from
clinically-defined patient populations will be used.  The results of
the immunohistochemical studies will be correlated with the
histopathologic diagnosis, tumor grade, stage of disease, response
to therapy, survival and other prognostic parameters of specific
significance for the neoplasms involved.

## Key facts

- **NIH application ID:** 3174971
- **Project number:** 2R01CA037194-04
- **Recipient organization:** CITY OF HOPE NATIONAL MEDICAL CENTER
- **Principal Investigator:** HECTOR A. BATTIFORA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $149,043
- **Award type:** 2
- **Project period:** 1984-04-01 → 1992-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3174971

## Citation

> US National Institutes of Health, RePORTER application 3174971, MONOCLONAL ANTIBODIES IN THE IMMUNOHISTOCHEMICAL CLASS (2R01CA037194-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3174971. Licensed CC0.

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