# HEPATITIS VIRUS & PRIMARY HEPATOCELLULAR CARCINOMA CELLS

> **NIH NIH R01** · WISTAR INSTITUTE · 1991 · $184,272

## Abstract

The relationship between HBV infection and the induction of
hepatocellular carcinoma is currently enigmatic.  We will explore
several mechanisms that might account for association of HBV
infection and liver tumor formation.  We have observed instability
in the genome of the host presenting with hepatocellular
carcinoma and in chronic carriers of HBV.  We therefore propose
to explore the possibilities that HBV might integrate in specific
areas of the host genome predisposed to instability that HBV
might induce such genome destablization or that environmental
factors or hereditary factors are at the root of the instability
observed.  Further, we propose to determine if genome instability
is of predictive value in these tumors.  We have established a
panel of hepatocellular carcinoma-derived cell lines of hepatocyte
morphology and function and plan to continue our analysis of their
common chromosome aberrations.  We propose to determine
specific breakpoints common to these cell lines to identify
specific areas of the genome which may contain genes responsible
for the control of hepatocyte proliferation.  By analysis of
somatic cell hybrids, derived from fusion of human hepatocellular
carcinoma-derived cell lines and stationary cultures of rat
hepatocytes, and transfection of the stationary rat hepatocyte
with cloned HBV and human hepatocellular carcinoma-derived
DNA, we plan to distinguish between the direct oncogenic
potenital of HBV and that of potentially activated human genes.
Furthermore, identifiecation of a gene(s) of a gene product(s) that
might effect such transformation is proposed.

## Key facts

- **NIH application ID:** 3175029
- **Project number:** 5R01CA037225-07
- **Recipient organization:** WISTAR INSTITUTE
- **SAM.gov UEI:** DW1XZMGNFBL4
- **Principal Investigator:** BARBARA B KNOWLES
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $184,272
- **Award type:** 5
- **Project period:** 1984-06-01 → 1992-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175029

## Citation

> US National Institutes of Health, RePORTER application 3175029, HEPATITIS VIRUS & PRIMARY HEPATOCELLULAR CARCINOMA CELLS (5R01CA037225-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3175029. Licensed CC0.

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