# MOLECULAR ANALYSIS OF CARCINOGEN-INDUCED DNA REPAIR

> **NIH NIH R01** · WASHINGTON UNIVERSITY · 1988 · $52,381

## Abstract

Excision repair is generally regarded as the major process by which
mammalian cells reduce the cytotoxic, mutagenic, and carcinogenic effects
of DNA damage produced by ultraviolet radiation and chemical carcinogens.
The long-term objective of this project is to investigate in detail the
molecular mechanisms of excision repair of carcinogen damage and the ways
in which the repair process is modulated by the chromatin structure of
mammalian cells.  Understanding the details of the excision repair process
is of particular interest in light of recent studies indicating that
different regions of mammalian genomes are repaired at different rates.
The first goal of the present application is to explore the role of ATP in
the early steps of excision repair in cells damaged with several different
carcinogens.  The possibility that the ATP requirement is related to
involvement of a DNA topoisomerase will be specifically examined.  This
application also proposes studies of damage and repair-associated
modifications of chromatin proteins.  Such modifications will be identified
using appropriate radioactively labeled precursors.  In addition, the
temporal relation of the chromatin modifications to the pre- and
post-incision phases of the repair process will be explored and the
physical relation of modified chromatin proteins to sites of excision
repair will be examined.  These studies will be conducted using a permeable
human fibroblasts in which excision repair proceeds in a fashion
essentially identical to that seen in intact cells, but is accessible at
all stages to biochemical manipulation.  The project also involves
development of a technique using biotin-modified dUTP and affinity
chromatography which for the first time will permit isolation and direct
analysis of chromatin undergoing excision repair.  The proposed studies
should generate biochemical data which will be directly applicable to
understanding the process of excision repair in vivo.

## Key facts

- **NIH application ID:** 3175105
- **Project number:** 5R01CA037261-04
- **Recipient organization:** WASHINGTON UNIVERSITY
- **SAM.gov UEI:** L6NFUM28LQM5
- **Principal Investigator:** STEVEN L DRESLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $52,381
- **Award type:** 5
- **Project period:** 1986-09-30 → 1989-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175105

## Citation

> US National Institutes of Health, RePORTER application 3175105, MOLECULAR ANALYSIS OF CARCINOGEN-INDUCED DNA REPAIR (5R01CA037261-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3175105. Licensed CC0.

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