# HEPATITIS B VIRUS DNA ONCOGENES AND LIVER CANCER

> **NIH NIH R01** · INSTITUT PASTEUR · 1987 · $81,284

## Abstract

This grant proposal falls into two parts.

We want to define as completely as possible the organization of integrated
hepatitis B viral DNA sequences in a number of hepatocellular carcinomas
(HCC).  We want to find out if there are common features such as sites of
integration, homologies between host and cellular sequences; whether or not
this cellular DNA is irreversibly deleted; if there are oncogenes in
flanking cellular sequences and if any HBV gene products are expressed in
HCC cells.  We will approach these questions primarily by molecular cloning
the subsequent analyses by blotting, subcloning, electron microscopy and
DNA sequencing.

Secondly based on our preliminary results we want to molecularly clone out
a cellular oncogene from HCC DNA that transforms NIH3T3 cells and find out
what the difference is to its normal allele.  Also we want to try
transfection other cells by newer transfection protocols.  In addition we
want to screen a number of HCCs (of different kinds) to determine whether
there is oncogene amplification, translocation orenhanced transcription.
These tasks will be approached by a combination of DNA transfection,
molecular cloning, DNA sequencing and DNA and RNA blotting.

## Key facts

- **NIH application ID:** 3175131
- **Project number:** 5R01CA037300-04
- **Recipient organization:** INSTITUT PASTEUR
- **SAM.gov UEI:** JRDEJNVPC8T3
- **Principal Investigator:** PIERRE TIOLLAIS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $81,284
- **Award type:** 5
- **Project period:** 1984-05-01 → 1988-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175131

## Citation

> US National Institutes of Health, RePORTER application 3175131, HEPATITIS B VIRUS DNA ONCOGENES AND LIVER CANCER (5R01CA037300-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3175131. Licensed CC0.

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