# CALORIES, ENERGY EXPENDITURE AND FISH OIL IN COLON CA.

> **NIH NIH R01** · INSTITUTE FOR CANCER PREVENTION · 1988 · $156,579

## Abstract

The specific aims of this ongoing study are (1) to determine the
efficacy of high fat diets containing various levels of Menhaden
fish oil and corn oil on azoxymethane (AOM)-induced colon
carcinogenesis in male F344 rats, and (2) to study the effects of
caloric restriction and energy expenditure on AOM-induced colon
carcinogenesis.

In order to study the effect of high dietary fish oil on the
initiating stage of colon carcinogenesis, groups of male F344 rats
(35 days old) will be fed a low or high fish oil diet and at 56 days
all animals will receive two weekly doses of AOM (15 mg/kg
bw/wk).  Two days later, groups of animals on high fish oil diet
will be transferred to a low fish oil diet, whereas animals on low
fish oil diet will be continued on the same diet.  All animals will
be necropsied at 36 weeks and colon tumor incidence compared.
In order to study the effect of various levels of dietary fish and
corn oil on post-initiating phase, groups of animals will be fed a
low-fat diet and 2 days after AOM treatment (15 mg AOM, once
weekly for 2 weeks) groups of animals will be transferred to high-
fat diets containing various levels of fish oil and corn oil and
continued on these diets until termination of experiment at 36
weeks.  All animals will be necropsied and colon tumor incidences
compared between various dietary treatments.  With regard to the
effects of caloric restriction and energy expenditure, 35 day old
male F344 rats will be fed a high fat diet ad libitum and at 56
days of age, animals will be treated with AOM as described above.
Two days later, groups of animals will be transferred to calorie-
restricted diets or exercise cages but fed high fat diet ad libitum
until termination of experiment at 36 weeks.  Colon tumor
incidence will be compared among the groups fed ad libitum and
calorie-restricted diets and those subjected to exercise.

## Key facts

- **NIH application ID:** 3175448
- **Project number:** 2R01CA037663-04A1
- **Recipient organization:** INSTITUTE FOR CANCER PREVENTION
- **Principal Investigator:** Bandaru S. Reddy
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $156,579
- **Award type:** 2
- **Project period:** 1984-07-15 → 1991-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175448

## Citation

> US National Institutes of Health, RePORTER application 3175448, CALORIES, ENERGY EXPENDITURE AND FISH OIL IN COLON CA. (2R01CA037663-04A1). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3175448. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*