# TYPES OF DIETARY FAT AND COLON CANCER

> **NIH NIH R01** · INSTITUTE FOR CANCER PREVENTION · 1993 · $197,736

## Abstract

The long-term objective is to understand the modulating effect of types
of dietary fat differing in fatty acid composition on colon cancer.
Specifically, it is proposed to study the mechanism(s) of types and
amount of fat namely corn oil (CO) and menhaden fish oil (FO) on colon
carcinogenesis.  Azoxymethane (AOM), an aliphatic azoxy compound will be
used to induce colon tumors.  The effect of types and amount of dietary
fat on the relevant biochemical parameters that are associated with colon
tumor promotion will be determined and will include cecal bacterial
7alpha-dehydroxylase and phospholipase C activities, fecal diglycerides
and bile acids, and colonic mucosal and tumor, 1,2-diacylglycerol,
phospholipase C, DAG kinase, protein kinase C, prostaglandin synthesis,
arachidonic acid metabolism via cyclooxygenase pathway, and ras p21.

At 5 weeks of age, male F344 rats will be fed low-fat AIN-76A diet.  At
7 weeks of age, groups of rats will be treated with two weekly s.c. doses
of AOM or normal saline (vehicle) and transferred to high fat diets
containing CO or FO diet.  Then, day at 1 and week 1 and 12 following AOM
treatment and at termination (wk 42), the above biochemical parameters
will be measured in colon mucosa and tumors (at termination) to elucidate
the mechanistic role of types and amount in colon carcinogenesis.  Also,
during the weeks of 4 and 12 on experimental diets and at termination,
groups of animals from each dietary group treated with AOM will be
sacrificed and cecal bacterial 7alpha-dehydroxylase and phospholipase C
and fecal bile acids and diglycerides will be determined.  Biochemical
and physiological parameters of various dietary groups will be analyzed
statistically to determine the significance of difference.  The selected
biochemical parameters taken together would suggest the possible
mechanism of in vivo tumor promotion or inhibition by types of dietary
fat.

## Key facts

- **NIH application ID:** 3175452
- **Project number:** 2R01CA037663-07A3
- **Recipient organization:** INSTITUTE FOR CANCER PREVENTION
- **Principal Investigator:** Bandaru S. Reddy
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $197,736
- **Award type:** 2
- **Project period:** 1993-09-30 → 1996-09-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175452

## Citation

> US National Institutes of Health, RePORTER application 3175452, TYPES OF DIETARY FAT AND COLON CANCER (2R01CA037663-07A3). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3175452. Licensed CC0.

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