# REGULATION OF EXPRESSION OF THE THYMIDINE KINASE GENE

> **NIH NIH R01** · LSU HEALTH SCIENCES CENTER · 1987 · $32,509

## Abstract

The long-term objective of this project is a complete understanding of the
modes of regulation of the thymidine kinase gene.  This includes cell-cycle
regulation, induction of normally quiescent cells by factors such as by
small DNA tumor viruses, and eventually its mode of repression in
terminally differentiated tissues such as heart and brain.  There are a
number of different health-related problems relevant to this.  One is that
DNA replication enzymes, including thymidine kinase, must be restimulated
for abnormal growth of cells such as in tumors.  Also, thymidine kinase
regulation is an important part of normal differentiation.  Lastly, in the
long term an understanding of how this gene is turned off in terminally
differentiated tissues may help to understand how to turn it and similar
genes back on, to regenerate damaged tissues in heart or nervous tissue.

The methodology will primarily center on the use of the cloned human cDNA
and gene for thymidine kinase.  Mutational studies will be used to
delineate the crucial transcriptional regulation sequences.  The regulatory
sequences in both the normal cell cycle and in papovavirus-induced
thymidine kinase activity will be compared.  Following the creation of a
thymidine kinase antisera, a complete analysis of RNA, protein, and enzyme
levels will be carried out.  This will help to define possible secondary
controlling influences in this system.  The complete primary structure of
this gene and its cDNA will be determined and studied in the process of
this work.  (N)

## Key facts

- **NIH application ID:** 3175472
- **Project number:** 3R01CA037673-03S1
- **Recipient organization:** LSU HEALTH SCIENCES CENTER
- **SAM.gov UEI:** M7KCJ79FAVH5
- **Principal Investigator:** Prescott L. Deininger
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $32,509
- **Award type:** 3
- **Project period:** 1984-07-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175472

## Citation

> US National Institutes of Health, RePORTER application 3175472, REGULATION OF EXPRESSION OF THE THYMIDINE KINASE GENE (3R01CA037673-03S1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3175472. Licensed CC0.

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