# OXIDATIVE DNA DAMAGE:  REPAIR AND CELLULAR RESPONSES

> **NIH NIH R01** · HARVARD UNIVERSITY · 1987 · $101,218

## Abstract

Ionizing radiation and oxidizing agents form many damages in common in
DNA.  Some of the lesions are potentially mutagenic or carcinogenic.
Little is currently known about whether or how most of these damages are
enzymatically repaired, or what other responses they prompt in living
cells.  This problem will be addressed by taking advantage of DNA
glycoslases that release specific products from oxidized DNA, and of a
newly discovered inducible resistance to oxidizing agents and ionizing
radiation.  The glycosylases and sensitive chemical assays will allow us to
assess the repair or persistence of a number of important oxidation lesions
in cellular DNA under a variety of conditions, and the relationship of
these to mutagenesis and toxicity.  The peroxide-induced resistance to
oxidation and radiation in E. coli is a key to determining the importance
of known enzymes in repair of oxidized DNA, and to locating and molecular
cloning of genes important in cellular recovery from oxidation and
radiation.  It will be investigated whether a similar induced response
exists in eukaryotic cells, notably yeast.  Known E. coli and yeast genes
will be assessed for their importance in constitutive or induced resistance
to oxidation.  It will also be tested whether oxidation induces promiscuous
or illegitimate recombinations.  The results will have a direct bearing on
deciding the biological effects of oxidation and of low-level radiation
damage to DNA.

## Key facts

- **NIH application ID:** 3175693
- **Project number:** 5R01CA037831-03
- **Recipient organization:** HARVARD UNIVERSITY
- **SAM.gov UEI:** LN53LCFJFL45
- **Principal Investigator:** Bruce F. Demple
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $101,218
- **Award type:** 5
- **Project period:** 1984-08-01 → 1989-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175693

## Citation

> US National Institutes of Health, RePORTER application 3175693, OXIDATIVE DNA DAMAGE:  REPAIR AND CELLULAR RESPONSES (5R01CA037831-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3175693. Licensed CC0.

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