# ISOTYPE, IDIOTYPE, AND ANTI-IDIOTYPE RESPONSES TO M-MULV

> **NIH NIH R01** · UNIVERSITY OF ALABAMA AT BIRMINGHAM · 1987 · $160,124

## Abstract

Host resistance to oncornavirus induced tumors is dependent on
both T-cell-mediated and humoral antibody immune mechanisms.
The experiments outlined in this proposal are designed to test the
hypothesis that antibodies can regulate cellular and humoral
immune responses to murine oncornaviral antigens through isotype
and idiotype specific reactions.  Immune responses of mice
against Moloney-murine sarcoma virus (M-MuSV) infection and M-
MuLV expressing lymphoma cells will be studied.  Determination
of the class and subclass of specific antibodies generated in
response to Moloney sarcomas and lymphomas may reveal key
information regarding why sarcomas regress in normal
nonimmunized mice but lymphomas do not even though they share
antigens.  We have generated several hybridomas secreting
monoclonal IgM antibodies that have specificity for an M-MuLV
antigen expressed on the cell surface.  The antibodies do not
neutralize virus but can inhibit M-MuSV induced tumor growth in
vivo.  These monoclonal antibodies and newly generated IgG
subclass monoclonal antibodies will also be tested for their ability
to inhibit syngeneic lymphoma growth.  Selected monoclonal
antibodies have been used to generate hybridomas secreting
monoclonal anti-idiotype antibodies (MAIDS).  The MAIDS will be
used to evaluate anti-idiotype regulation of immune responses to
M-MuSV infection and M-MuLV induced tumors.  Thus, we will
attempt to modulate idiotype specific responses in animals in the
presence and absence of virus induced tumor antigens by
administering MAIDS or monoclonal antibodies with MuLV
specificity.  The monoclonal antibodies will also be used to search
for idiotype or anti-idiotype positive T cells generated during an
immune response to M-MuLV specific antigens.  These studies
should provide information that will be conceptually useful in our
understanding of immunity to oncornavirus infection and suggest
potential strategies for prevention or therapy of virus induced
tumors.

## Key facts

- **NIH application ID:** 3175990
- **Project number:** 1R01CA038008-01A3
- **Recipient organization:** UNIVERSITY OF ALABAMA AT BIRMINGHAM
- **SAM.gov UEI:** YND4PLMC9AN7
- **Principal Investigator:** EDDIE W LAMON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $160,124
- **Award type:** 1
- **Project period:** 1987-08-19 → 1990-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3175990

## Citation

> US National Institutes of Health, RePORTER application 3175990, ISOTYPE, IDIOTYPE, AND ANTI-IDIOTYPE RESPONSES TO M-MULV (1R01CA038008-01A3). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3175990. Licensed CC0.

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