# DNA REARRANGEMENTS IN MOMULV INDUCED THYMOMAS

> **NIH NIH R01** · FOX CHASE CANCER CENTER · 1990 · $212,261

## Abstract

Our studies of oncogenesis in MoMuLV induced rat thymic
lymphomas have detected four putative oncogenes (Mlvi-1, Mlvi-3
and Mlvi-4) which appear to be involved in this process.
Furthermore, in recent studies aiming at the dissection of the
genetic events that occur in the early (tumor induction) and late
(tumor progression) stages of oncogenesis we have shown that:  a)
the spleens of preleukemic animals contain populations of cells
that are clonal with regard to provirus insertion, and b) tumor
progression is associated with provirus reintegration in tumor cell
DNA. The objectives of this work are:  a) to determine the role of
loci targeted by provirus integration during oncogenesis, b) to
determine the mechanism and the significance of provirus
reintegration during tumor progression and c) to exploit provirus
integration as a marker to determine the nature and fate of
preleukemic cells and the mechanism of segregation of leukemic
cells in a single tumor bearing animal.  The study of the molecular
events involved in the induction and progression of rat thymic
lymphomas may provide information directly applicable to the
understanding of the biology of human lymphoid neoplasms.

The work proposed here aims at:

I.  The characterization of the Moloney leukemia virus integration
(MLvi) loci in the rat and

II.  The analysis of the role of provirus integration in tumor
induction and progression.

To achieve these goals we will utilize the following methodology:
Southern and Northern blotting, pulse field gradient gel
electrophoresis, cloning of genomic DNA and cDNA, DNA
sequence analyses, immune precipitation and Western blotting, S1
and primer extension mapping of mRNA transcripts, raising of
antibodies, transfection, electroporation, transplantation of
preleukemic and leukemic cells into recipient animals, and culture
of normal and tumor cells.

## Key facts

- **NIH application ID:** 3176073
- **Project number:** 5R01CA038047-06
- **Recipient organization:** FOX CHASE CANCER CENTER
- **Principal Investigator:** PHILIP N. TSICHLIS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $212,261
- **Award type:** 5
- **Project period:** 1984-07-01 → 1993-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176073

## Citation

> US National Institutes of Health, RePORTER application 3176073, DNA REARRANGEMENTS IN MOMULV INDUCED THYMOMAS (5R01CA038047-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176073. Licensed CC0.

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