# NUCLEOTIDE BINDING PROPERTIES OF SV40 LARGE T PROTEIN

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1989 · $178,258

## Abstract

The long-term goal of this project is to determine the location
and parameters of the nucleotide binding site(s) in SV40 large T
antigen (T-Ag), and investigate the relationship between ATPase,
adenylylation and other functions of T-Ag.  Since there are
several activities involving nucleotide associated with the protein,
we will first determine whether there is one or more binding sites
for ATP and the binding affinity(s).  We have proposed a
topological model for a consensus ATP binding domain in the
protein, without the aid of crystallographic analyses, which
includes the active sites for ATPase and adenylation.  The
research plan will focus on testing aspects of this newly proposed
model both biochemically and genetically.  At least two reactive
ATP analogs will be used to probe the ATP binding site in T-Ag,
the modifications will be analyzed for their specificity, and the
modified residues will be mapped.  Using the model to facilitate
the targets for mutagenesis, the ATP binding site will also be
probed genetically.  Each of the mutant T-Ags will be tested for
its ability to bind and hydrolyze ATP, and to be adenylylated.  The
results of these studies might support aspects of the proposed
model or refute them, and the biochemical properties of the
mutant T-Ags could reveal whether there is a relationship
between nucleotide ATPase and adenylylation.  With the relevant
T-Ag mutants, further investigation of the biochemical and/or
functional relationship between nucleotide binding activities and
other associated functions of the protein will be investigated such
as helicase, oligomerization, autoregulation of viral mRNA
synthesis, oncogenic transformation and SV40 DNA replication.
These investigations are intended to help in resolving the
individual functions of T-Ag from one another and to help in
understanding the details of T-Ag's interactions with the infected-
cell.

## Key facts

- **NIH application ID:** 3176091
- **Project number:** 5R01CA038069-06
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** MARGARET K BRADLEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $178,258
- **Award type:** 5
- **Project period:** 1984-07-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176091

## Citation

> US National Institutes of Health, RePORTER application 3176091, NUCLEOTIDE BINDING PROPERTIES OF SV40 LARGE T PROTEIN (5R01CA038069-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176091. Licensed CC0.

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