# TUMOR INHIBITORY FACTORS IN COLON CARCINOMA

> **NIH NIH R01** · BAYLOR COLLEGE OF MEDICINE · 1989 · $85,913

## Abstract

Polypeptide factors which stimulate or inhibit cell growth have
been identified in many normal and neoplastic tissues and cells.
We have identified and purified 2 inhibitory peptides called tumor
inhibitory factors (TIF) and transforming growth factor-B (TGF-B)
from a human colon carcinoma cell line.  The long term goals of
this project are to understand the mechanisms by which these 3
factors inhibit cell growth.  These experiments will utilize two
model systems: a bank of human colon carcinoma cell lines which
vary in their growth and biological properties and a transformed
and nontransformed mouse embryo fibroblast cell line.  The level
of 2 TIF's and TGF-B produced by color carcinoma cell lines (some
of which are responsive and others non-responsive to these factors)
will be examined to determine if there is a correlation between the
factors produced and the sensitivity of the cells to these factors.
The ability of these factors to affect DNA synthesis of the mouse
fibroblast cell line will be determined.  The effect of
differentiation agents on the types of growth factors produced  by
the colon cells and their ability to induce DNA synthesis in
fibroblasts will also be examined.  One differentiation agent
blocks the induction of DNA synthesis by TGF-B in fibroblasts.  In
order to determine at which step of the mitogenic cascade this
occurs, the effect on TGF-B binding to its receptor and expression
of platelet-derived growth factor and its mRNA will be determined.
An assay system utilizing the colon carcinoma cell lines will be
established to answer similar questions.  This project will provide
information concerning the mechanism of growth control in normal
and malignant cells.  This may lead to the design of agents which
may be of therapeutic use in halting malignant cell growth.

## Key facts

- **NIH application ID:** 3176119
- **Project number:** 5R01CA038100-05
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **SAM.gov UEI:** FXKMA43NTV21
- **Principal Investigator:** ALAN E LEVINE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $85,913
- **Award type:** 5
- **Project period:** 1988-03-01 → 1991-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176119

## Citation

> US National Institutes of Health, RePORTER application 3176119, TUMOR INHIBITORY FACTORS IN COLON CARCINOMA (5R01CA038100-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176119. Licensed CC0.

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