# CHEMISTRY OF NOVEL NATURAL INHIBITORS OF CARCINOGENESIS

> **NIH NIH R01** · OHIO STATE UNIVERSITY · 1991 · $184,877

## Abstract

The objective of this application is to isolate and study the
chemistry and mechanism of action of novel inhibitors of
carcinogenesis form higher plants using a bioassay based on B(a)P
metabolism.  These mammalian cell culture assays detect the
inhibitors of interest in a variety of human foods shown to be
correlated to reduced cancer incidence.

Specific aims include 1) Acquisition of additional higher plants
for initial screening in the B(a)P metabolism assay to establish
active leads; 2) Bioassay-directed fractionation of plants
including members of the Cruciferae, Umbelliferae, Leguminosae,
and other s which shown reproducible activity in the bioassays in
order to isolate pure, crystalline active inhibitory components.
Specific projects underway involve isolation and characterization
of active compounds from Trifolium pratense and Coffea arabica.
These fractionations will also be guided by assays of the effect
of test compounds on benzo(a)pyrene metabolism in hamster embryo
cell cultures; 3) Determinations of the complete structure and
stereochemistry of crystalline actives using modern spectroscopic
(MS), and X-ray crystallography; 4) Determination of the mechanism
of action of selected active components with high chemopreventive
potential.  These studies will include hydrocarbon metabolism,
hydrocarbon-DNA interactions and hydrocarbon induced mutation in
mammalian systems; and 5) Development of analytical methods
including HPLC, GC/MS, and MS/MS to detect these compounds at low
levels in biological systems and other plants for purposes of
dereplication, identification of other inhibitors, and other plant
sources of inhibitors.  The identification of these novel
inhibitors will lead to a variety of agents with the potential of
preventing human cancer, and in addition will provide a better
understanding of the total inhibitor activity present in the
plants, and their mechanism of action.

## Key facts

- **NIH application ID:** 3176200
- **Project number:** 5R01CA038151-07
- **Recipient organization:** OHIO STATE UNIVERSITY
- **SAM.gov UEI:** DLWBSLWAJWR1
- **Principal Investigator:** JOHN M CASSADY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $184,877
- **Award type:** 5
- **Project period:** 1984-07-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176200

## Citation

> US National Institutes of Health, RePORTER application 3176200, CHEMISTRY OF NOVEL NATURAL INHIBITORS OF CARCINOGENESIS (5R01CA038151-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176200. Licensed CC0.

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