# BONE-BONE MARROW INTERACTION

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1988 · $134,018

## Abstract

The long term objective of this research proposal is to increase
our knowledge in physiological interrelationships between
hemopoietic tissue and bone tissue, seeking a possible role of
hemopoietic cells in regulation of bone turnover.  Using an animal
del of tumor-induced granulocytosis associated with hypercalcemia,
and isolating the more derived humoral factors, the mechanisms of
bone resorption in hyperplastic bone arrow will be explored.
During the next grant period, the investigation of bone cell
stimulating and bone resorbing humoral agents demonstrated from the
CE mammary carcinoma cell clones will be investigated for their
molecular relationships to granulopoieticc growth factors also
elaborated from the tumor, by the use of molecular probes, well as
specific antisera of hemopoietic growth factors, conventional
biochemical purification procedures and analysis of gene
expression.  A new method to precisely analyses osteoclast
progenitors from the bone marrow in vitro will be established,
characterized and utilized for assays of bone resorbing activities.
The in vivo function of isolated CE tumor derived bone modulating
or granulopoietic factor will be tested in vivo for its ability to
care osteopetrosis in mice.  The role of the CE mammary carcinoma-
derived factor as well as various purified hemopoietic growth
factors in bone resorbing process will be explored by in vitro
culture experiments using isolated bone forming cells, marrow
stromal cells, marrow mononuclear cells and bone particles and the
mechanism of growth factor mediated bone resorotion will be
dissected.  These studies are to disclose many unanswered basic
questions about the defect of hemopoietic growth factors on
physiology of bone marrow and bone turnover; well as to understand
pathophysiology of unexplained hypercalcemia associated with
certain tumors.

## Key facts

- **NIH application ID:** 3176252
- **Project number:** 2R01CA038189-04A1
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** MINAKO Y LEE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $134,018
- **Award type:** 2
- **Project period:** 1984-06-15 → 1992-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176252

## Citation

> US National Institutes of Health, RePORTER application 3176252, BONE-BONE MARROW INTERACTION (2R01CA038189-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176252. Licensed CC0.

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