# CALCIUM FLUX IN PITUITARY  CELLS

> **NIH NIH R01** · UNIVERSITY OF VIRGINIA · 1992 · $160,797

## Abstract

Cellular calcium has a pivotal regulatory role in many aspects of: 1)
normal physiology, such as hormone secretion, control of other
intracellular messengers and modulation of cancer-promoting oncogenes;
and 2) human disease (calcium is a pathogenic factor in the neuronal
damage due to stroke, epilepsy and hypoglycemia). With hormone secretion
from rat anterior pituitary cells as the model system, the broad
objective of this program is to learn how normal cells control calcium
ion movements and calcium concentration and compare this knowledge with
observations in cancer cells where calcium-dependent activities are
abnormal. Endogenous hypothalamic hormones or selected pharmacologic
agents will be applied to cultured normal or neoplastic anterior
pituitary cells to perturb the intrinsic systems modulating calcium flux
as the cell executes its biological role of hormone secretion. The
intracellular calcium concentrations will be measured with an
intracellular fluorescent probe, Indo-1, during perifusion of the
pituitary cells to allow concomitant analysis of the hormone secretory
rate. These investigations will shed light on the normal mechanisms
governing calcium ion flux and the alterations that confer neoplastic
properties upon certain cells.

The experimental procedures required to complete this project are
established and the preliminary work demonstrates feasibility.

## Key facts

- **NIH application ID:** 3176317
- **Project number:** 2R01CA038228-04A4
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **SAM.gov UEI:** JJG6HU8PA4S5
- **Principal Investigator:** IVAN S LOGIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $160,797
- **Award type:** 2
- **Project period:** 1985-12-01 → 1996-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176317

## Citation

> US National Institutes of Health, RePORTER application 3176317, CALCIUM FLUX IN PITUITARY  CELLS (2R01CA038228-04A4). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176317. Licensed CC0.

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