# MAMMALIAN CELL TRANSFORMATION BY ONCOGENIC VIRUSES

> **NIH NIH R01** · UNIVERSITY OF TEXAS AT AUSTIN · 1987 · $141,516

## Abstract

The long term objective of these studies is to define DNA
sequences and protein structural elements responsible for
polyomavirus transcription and DNA replication and to explain
how they function in animal cells.  In previous years we have
isolated and partially characterized polyomavirus mutants with
alterations in the early promoter, in the origin of DNA replication
and in the enhancer.  Our specific aims for the forthcoming years
are to use these mutants to (a) define structural features of the
polyomavirus large T-antigen which are responsible for sequence-
specific recognition of the origin; (b) identify domains of the
polyomavirus origin which are required for DNA replication and
define how they function; (c) define DNA sequence elements
within the polyomavirus enhancer which activate transcription
and/or replication; (d) characterize cellular proteins which bind to
the enhancer, and which are required for DNA replication and/or
transcription; and (e) reconstitute in vitro transcription and DNA
replication systems which depend upon the enchancer for activity,
and determine at what steps the enhancer binding proteins
participate.

## Key facts

- **NIH application ID:** 3176585
- **Project number:** 2R01CA038538-04A1
- **Recipient organization:** UNIVERSITY OF TEXAS AT AUSTIN
- **SAM.gov UEI:** V6AFQPN18437
- **Principal Investigator:** William Robert Folk
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $141,516
- **Award type:** 2
- **Project period:** 1984-09-01 → 1992-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176585

## Citation

> US National Institutes of Health, RePORTER application 3176585, MAMMALIAN CELL TRANSFORMATION BY ONCOGENIC VIRUSES (2R01CA038538-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176585. Licensed CC0.

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