# MAMMALIAN CELL TRANSFORMATION BY ONCOGENIC VIRUSES

> **NIH NIH R01** · UNIVERSITY OF MISSOURI-COLUMBIA · 1993 · $197,678

## Abstract

DESCRIPTION (Adapted from the applicant's abstract):  Aberrant control
of the  initiation of cellular DNA replication leads to cancer.The
purpose of the  studies proposed in this application is to gain insight
into the molecular  mechanisms governing the initiation of DNA
replication in mammalian cells,  with the expectation that this knowledge
will provide the means to reduce  proliferation of neoplastic cells.

The small DNA tumor viruses provide excellent experimental models with
which to study DNA replication in mammalian cells.  These viruses depend
largely  upon cellular machinery to replicate their genomes, and now much
of the  biochemistry of the replication complex that is assembled with
viral and  cellular proteins is understood.  However, it is not
understood how the  assembly of this complex and its subsequent activity
is regulated, and this  knowledge should provide insights into how
cellular replication is regulated.  In these studies Dr. Folk proposes
to utilize in vitro and in vivo replication  assays with purified
proteins and DNAs to focus upon two aspects of the  regulation of DNA
replication of the murine polyomaviruses:  1) The role of  the viral
tumor antigens in modifying cellular proteins and cellular signaling
pathways to render the host cell permissive for DNA replication. In
particular, the importance of a novel viral protein containing sequences
capable of interacting with DNA polymerase-DNA primase will be explored.
2) The role of cellular proteins which bind the polyomavirus enhancer in
activating the viral origin and the replication complex assembled
thereupon.  In particular, Dr. Folk will focus upon the AP1 protein,
which is a paradigm  for cellular transcription factors, and ets family
proteins binding to the  PEA3 motif.

## Key facts

- **NIH application ID:** 3176586
- **Project number:** 2R01CA038538-09
- **Recipient organization:** UNIVERSITY OF MISSOURI-COLUMBIA
- **SAM.gov UEI:** SZPJL5ZRCLF4
- **Principal Investigator:** William Robert Folk
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $197,678
- **Award type:** 2
- **Project period:** 1984-09-01 → 1997-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176586

## Citation

> US National Institutes of Health, RePORTER application 3176586, MAMMALIAN CELL TRANSFORMATION BY ONCOGENIC VIRUSES (2R01CA038538-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176586. Licensed CC0.

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