# C-MYC ONCOGENE REGULATION IN NORMAL AND MALIGNANT CELLS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1987 · $81,712

## Abstract

These studies focus on regulation of the murine c-myc oncogene gene, the
cellular homologue of the MC29 virus transforming gene.  There is evidence
that c-myc expression is highly regulated in normal cells where its
induction correlates with an early phase of mitogen activation and that
alterations in c-myc expresion can play a causal role in malignant
transformation.  Accordingly, elucidation of the regulation, as well as the
function, of the c-myc gene appears to be a key element for understanding
normal growth control and malignant transformation.  The proposed
experiments will characterize the regulation of both normal c-myc gene
expression and the aberrant c-myc gene expression which occurs after
translocation of the c-myc gene in murine plasmacytomas.

These studies are divided into three parts.  The first part centers on
transcriptional regulation of the normal c-myc gene.  The studies will show
if c-myc repression occurs when cellular levels of myc protein are elevated
and if myc protein autoregulates the c-myc gene.  The c-myc gene region
required for its repression will be mapped.  The ability of 1,25 dihydroxy
vitamin D3 to repress c-myc transcription will also be confirmed and
studied.  In the second part of the study the mechanism(s) responsible for
activating transcription of translocated c-myc genes from cryptic promoters
in murine plasmacytomas will be characterized.  These experiments will
demonstrate if DNA sequences from the immunoglobulin region are necessary
for c-myc activation; if so, the region will be mapped and the tissue
specificity of the activation will be explored.  In the final portion of
the work, steady state levels and stability of c-myc mRNA and myc protein
in quiescent normal cells, mitogen induced cells and plasmacytoma tumors
will be compared.  (X)

## Key facts

- **NIH application ID:** 3176627
- **Project number:** 5R01CA038571-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** KATHRYN L. CALAME
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $81,712
- **Award type:** 5
- **Project period:** 1985-09-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176627

## Citation

> US National Institutes of Health, RePORTER application 3176627, C-MYC ONCOGENE REGULATION IN NORMAL AND MALIGNANT CELLS (5R01CA038571-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176627. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*