# GENETIC STUDIES OF HUMAN B CELL CANCER

> **NIH NIH R01** · STANFORD UNIVERSITY · 1987 · $171,329

## Abstract

This research focuses on the application of several recently developed
molecular genetic techniques to the analysis of clinical and basic problems
related to B cell lymphoma and other B lymphocytic neoplasms.  A number of
projects pertain to immunoglobulin (Ig) gene rearrangements in the
diagnosis of B cell neoplasms:  (1) Analysis of clonal Ig gene
rearrangements will be used to determine whether detection of
rearrangements will be useful or a diagnostic criterion for all cases of B
cell lymphoma, including cases lacking cellular immunoglobulin.  (2)\DNA
from null cell lymphomas will be examined for Ig gene rearrangements to
determine what percentage of these cases are of B cell lineage.  (3)
Lymphoid tissue from patients with idiopathic thrombocytopenic purpura will
be tested for clonal Ig gene rearrangements to ensure that monoclonal
immune responses occur rarely, or perhaps never, in an autoimmune disease.
(4)\Peripheral blood and bone marrow will be screened for clonal Ig gene
rearrangements in an effort to detect small numbers of lymphoma cells
migrating in the blood, or to monitor minor populations of residual or
reemerging neoplastic B cells in the bone marrow of treated patients.  A
second major portion of this research concerns establishing the incidence
of bi-\and multiclonal B cell lymphomas in a variety of clinical settings.
Evidence for a possible common progenitor stem cell in these cases will be
sought by detecting inactivation of the same X chromosome among the
multiple tumors of disparate clonal origin from individual female
patients.  X-inactivation in these clones will be studied by a new approach
based on DNA polymorphisms and differences in chromatin structure between
active and inactive X chromosomes.  Finally, cultured cell lines will be
generated from lymphoproliferative lesions arising in immunosuppressed
organ transplant recipients.  Karyotype and drug sensitivity studies will
be performed on these cells, and DNA extracted from these cells and the
tumors from which they were originally derived will be utilized in in vitro
transformation experiments to identify putative activated oncogenes.  (6)

## Key facts

- **NIH application ID:** 3176708
- **Project number:** 5R01CA038621-03
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** Jeffrey Sklar
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $171,329
- **Award type:** 5
- **Project period:** 1985-01-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176708

## Citation

> US National Institutes of Health, RePORTER application 3176708, GENETIC STUDIES OF HUMAN B CELL CANCER (5R01CA038621-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176708. Licensed CC0.

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