# GENETIC STUDIES OF HUMAN B CELL CANCER

> **NIH NIH R01** · STANFORD UNIVERSITY · 1989 · $45,335

## Abstract

Research proposed in this application will investigate a number of
genetic problems directly or indirectly related to human B cell
neoplasia and having both clinical and basic biologic
ramifications.  Five projects are planned.  These involve the
following:  (1) Analysis of the multi-clonality of
lymphoproliferative lesions in immunosuppressed patients.  This
project will utilize restriction fragment length polymorphisms of
the fused termini of Epstein-Barr virus genomes as markers of
clonal cellular proliferation to determine whether or not separate
lymphoproliferative lesions in individual patients arise from
independent transformation events.  (2) Analysis of human non-
immunoglobulin gene DNA which contains strong sequence
homology to DNA of the immunoglobulin heavy chain enhancer
region.  In addition to cloning, sequencing, and mapping this DNA
on the human genome, studies will be carried out to determine
possible functions of this DNA and its flanking sequences.  (3)
Molecular cloning and structural analysis of DNA corresponding to
cytologic breakpoints involved in the t(4;11)(q21;q23)
chromosomal translocation of common acute lymphocytic
leukemia.  DNA sequences cloned in this project will be used as
hybridization probes to analyze sites of rearrangement and
transcription in human B cell tumors.  (4) Investigation of somatic
hypermutation in rearranged immunoglobulin genes of B cell
lymphomas and cell lines.  The basic biology of somatic mutation
will be studied by cloning and sequencing immunoglobulin genes
and cDNA from tumors and cultured cells.  Attempts will also be
made to develop an in vitro tissue culture system for analysis of
somatic mutation in both endogenous and transfected
immunoglobulin genes.  (5) Investigation of the possible evolution
of multiple myeloma from myeloma-related clonal B cells.
Immunoglobulin DNA cloned from bone marrow and blood
specimens of myeloma patients will be analyzed to determine
whether or not the neoplastic plasma cells in this disease develop
by a multistep mechanism involving clonal B cells.  In addition,
this project will investigate somatic mutation in the
immunoglobulin genes of plasma cells and their late B cell
precursors.

## Key facts

- **NIH application ID:** 3176709
- **Project number:** 5R01CA038621-05
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** Jeffrey Sklar
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $45,335
- **Award type:** 5
- **Project period:** 1985-01-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176709

## Citation

> US National Institutes of Health, RePORTER application 3176709, GENETIC STUDIES OF HUMAN B CELL CANCER (5R01CA038621-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176709. Licensed CC0.

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