# ONC GENE INTRODUCTION AND EXPRESSION

> **NIH NIH R01** · UNIVERSITY OF PENNSYLVANIA · 1988 · $203,440

## Abstract

We have been successful in the last few years in introducing new genes into
the germ line of mice.  A significant number of these new genes have been
expressed in the animals and their progeny.  The present study involves the
introduction of onc genes into mice.  Many of the studies will be done with
the SV40 early region which contains the large T and small t antigen
genes.  We have found this region will induce choroid plexus adenomas and
carcinomas in transgenic mice.  The genes are also expressed in other
tissues of the animal.  This provides a novel and exciting model in which
to study the mechanism of oncogenesis.  We wish to investigate the:  (1)
events associated with activation of the gene (e.g., DNA methylation,
amplification, rearrangement); (2) role of the large T and small t coding
sequences in tumor development; (3) role of enhancer elements in the
frequency and target cell specificity of tumor development; (4) effects of
other onc genes, such as src and ras; and (5) effect of carcinogenic agents
on transgenic mice that contain onc genes but do not show obvious signs of
malignancy.  The ability to introduce known oncogenic DNA sequences into an
animal and study the effect of the genes provides an excellent experimental
system for studying cancer.  The experiments in animals are particularly
important for the identification of DNA changes associated in vivo with
gene activation and factors controlling tissue-specific expression.  (X)

## Key facts

- **NIH application ID:** 3176740
- **Project number:** 5R01CA038635-04
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **SAM.gov UEI:** GM1XX56LEP58
- **Principal Investigator:** Ralph Lawrence Brinster
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $203,440
- **Award type:** 5
- **Project period:** 1985-01-01 → 1989-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176740

## Citation

> US National Institutes of Health, RePORTER application 3176740, ONC GENE INTRODUCTION AND EXPRESSION (5R01CA038635-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176740. Licensed CC0.

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