# CYTOCHROME P-450 GENE STRUCTURE AND REGULATION

> **NIH NIH R01** · MAHARISHI UNIVERSITY OF MANAGEMENT · 1987 · $171,356

## Abstract

The PAH-induced or MC-induced P-450s are responsible for the metabolism of
carcinogens, as well as a wide variety of both endogenous and exogenous
compounds.  The metabolism of carcinogens by PAh-induced P-450s can occur
via pathways that contribute to their detoxification, or via pathways that
convert these compounds to highly active carcinogenic species.  However,
several important questions about the process of chemical carcinogenesis
and the metabolism of carcinogens by the P-450s remain to be answered.
Many of these questions can be most effectively addressed through the
analysis of the structure and regulation of the genes that encode the
PAH-induced P-450s.  The proposed research is designed to provide basic
knowledge oif the structure and regulation of the P-450 genes.  This
knowledge combined with the cloned P-450 cDNAs and genes that will be
generated during this project will provide tools required to assess the
role of specific P-450 gene products in carcinogenesis, and to determine
individual variation in susceptibility to cancer.  The specific objectives
of this research are to:

Carry out the detailed structural analysis of two PAH-induced P-450 genes,
that are available only in the PI's laboratory, and compare the structures
of these genes to the published structures of other members of this gene
family.

Investigate other aspects of P-450 gene structure that will yield
information for the analysis of P-450 gene regulation and for investigating
the roles of specific P-450 gene products in carcinogenesis

Study the roles that modulation of transcription, mRNA stabilization and
mRNA processing play in regulating P-450c, P-450d and P-450m mRNA levels in
vivo and in a cultured hepatocyte cell line

Use DNA-mediated gene transfer of cloned P-450 genes, gene fragments and
gene hybrids to locate sequences that take part in the control of P-450c,
P-450d and P-450m gene expression.

## Key facts

- **NIH application ID:** 3176805
- **Project number:** 5R01CA038655-02
- **Recipient organization:** MAHARISHI UNIVERSITY OF MANAGEMENT
- **SAM.gov UEI:** P78NHUPVXCB4
- **Principal Investigator:** JOHN B FAGAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $171,356
- **Award type:** 5
- **Project period:** 1986-07-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176805

## Citation

> US National Institutes of Health, RePORTER application 3176805, CYTOCHROME P-450 GENE STRUCTURE AND REGULATION (5R01CA038655-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176805. Licensed CC0.

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