# TRANSFORMING SEQUENCES OF YABA VIRUS DNA

> **NIH NIH R01** · SOUTHERN ILLINOIS UNIVERSITY CARBONDALE · 1989 · $108,276

## Abstract

This proposal is concerned with the characterization of the
transforming DNA sequences of Yaba monkey tumor pox virus.
Study of the cause and effect relationship between the presence
of viral DNA sequences, their expression, and the transformed
state of the cells will be made using revertants to test for the
presence of viral DNA sequences and using DNA from
transformed cells to transform other cells.  Identification of viral
DNA sequences responsible for transformation will be made
initially by screening independently transformed cell lines for
common DNA segments and by serial transformation with viral
DNA segments isolated from transformed cells on the theory that
serial transformation will lead to loss of unlined, nonessential
viral DNA sequences in transformed cells.  The physical state of
Yaba virus specific DNA in the nucleus of transformed cells will
be characterized as existing free in the nucleus or integrated into
the cellular DNA or both.  Fine mapping of viral DNA sequences
found in transformed cells will be studied by subcloning cloned
DNA fragments found in transformed cells and using Northern
blot hybridization and in vitro translation of identified
messengers.  Proteins synthesized will be compared to those in
transformed cells.  The role of viral DNA in the transfection of
cells will be studied by neoplastic transformation of Balb/c 3T3
fragments of Yaba virus DNA will be used to identify Yaba DNA
sequences in the genome of transformed cells.  The possibility
that the transforming DNA sequences of Yaba virus are found in
other pox viruses will be investigated.  Genetic expression of
Yaba virus DNA sequences found in transformed cells will be
studied.  Cells transformed with Yaba virus will be tested for
their ability to form tumors in hamsters, mice and monkeys.
Characterization of the transforming DNA sequences of Yaba
virus should elucidate additional information that will lead to the
identification of the mechanism(s) of transformation by Yaba
virus.  This information is of particular interest because Yaba
virus is oncogenic in monkeys, its natural host, and it produces
tumors in man.

## Key facts

- **NIH application ID:** 3176861
- **Project number:** 5R01CA038678-02
- **Recipient organization:** SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
- **SAM.gov UEI:** Y28BEBJ4MNU7
- **Principal Investigator:** HASSAN ROUHANDEH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $108,276
- **Award type:** 5
- **Project period:** 1987-01-01 → 1990-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3176861

## Citation

> US National Institutes of Health, RePORTER application 3176861, TRANSFORMING SEQUENCES OF YABA VIRUS DNA (5R01CA038678-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3176861. Licensed CC0.

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