# VIRAL INDUCED TUMORIGENESIS

> **NIH NIH R01** · PRINCETON UNIVERSITY · 1989 · $225,230

## Abstract

Two experimental protocols will be employed to examine the role of gene
enhancer signals in regulating gene expression of viruses in a tissue
restricted fashion.  1. Transgenic mice with SV40 DNA in all cells and the
germ line have been obtained.  With several independently derived mice of
this type there is a tissue restricted or preferred gene expression of SV40
mRNA and tumor antigen (brain, thymus, kidney).  Similarly there is a
tissue limited or preferred SV40 induced tumorigenesis in these transgenic
mice.  Experiments have been designed to determine which genetic elements
of SV40 DNA (gene enhancer, T-antigen, t-antigen) are responsible for
tissue limited gene expression and/or tumorigenesis in these transgenic
mice.  Transgenic mice containing viral "immortality" gene functions of
polyoma, adenovirus and SV40 will be obtained and the role of these gene
products in tumorigenesis will be studied.  Permanent cell lines derived
from a variety of tissues of these transgenic mice can be useful to study
developmental questions or detect possible oncogenes.  2. The role of the
polyoma gene enhancer sequences in regulating gene expression in embryonal
carcinoma cells and a variety of differentiated cell types will be
studied.  Several methods for obtaining gene enhancer mutants with a cell
or tissue preference are described.  Such gene enhancer mutants should be
useful in experiments with transgenic mice.

## Key facts

- **NIH application ID:** 3177010
- **Project number:** 5R01CA038757-05
- **Recipient organization:** PRINCETON UNIVERSITY
- **SAM.gov UEI:** NJ1YPQXQG7U5
- **Principal Investigator:** ARNOLD J LEVINE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $225,230
- **Award type:** 5
- **Project period:** 1984-12-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177010

## Citation

> US National Institutes of Health, RePORTER application 3177010, VIRAL INDUCED TUMORIGENESIS (5R01CA038757-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177010. Licensed CC0.

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