# ROLES OF POLYPEPTIDE GROWTH FACTORS IN CELL GROWTH

> **NIH NIH R01** · SAINT LOUIS UNIVERSITY · 1993 · $282,349

## Abstract

DESCRIPTION (Adapted from the investigator's abstract):  The investigator
plans to continue his studies into the roles of the polypeptide growth
factors, platelet-derived growth factor (PDGF), acidic fibroblast growth
factor (aFGF), and transforming growth factor-beta (TGF-beta) and their
receptors in the regulation of growth of normal and transformed cells.
The four specific aims are as follows:  (1)  To define the mechanism of
intracellular loop autocrine transformation in cells transformed by v-sis
and c-sis (PDGF-B).  The signal transduction mediated by the
intracellular, activated PDGF receptors will be studied by determination
of the kinase activities of the intracellular activated PDGF receptors
and by examination of the potential interaction of the intracellular
activated PDGF receptors with phospholipase Cgamma, pp60 c-src, PI-3
kinase, or GTPase activating proteins. (2) To purify and characterize the
cell surface binding protein(CSBP) for sis gene products from cultured
fibroblasts and bovine liver membranes, to investigate the cell biology
of CSBP, and to examine the effect of CSBP expression on the
tumorigenicity of sis-transformed cells.  CSBP will be purified from
crude plasma membranes of cultured fibroblasts and bovine liver. The
biosynthesis, processing and turnover of CSBP in cultured cells will be
studied using specific anti-CSBP antibodies. (3)To characterize the
Ser/Thr-specific protein kinase activity of bovine liver major aFGF-
stimulated phosphoprotein (MAFP), a newly discovered ecto-protein kinase,
and to examine the cell biology of MAFP.  The MAFP-mediation of the
mitogenic action of aFGF and its effect on cellular signalling will be
examined in cells that do not express the aFGF receptor/protein tyrosine
kinase. (4)  To characterize the Ser/Thr-specific protein kinase activity
of the type V TGF-beta receptor and to study the cell biology and
molecular biology of this newly discovered receptor.  The kinase activity
of the type V TGF-beta receptor will be studied with respect to substrate
specificity and identification of the autophosphorylation site and
physiological substrates.  The cloning from a bovine cDNA library and the
sequencing and expression of the type V TGF-beta receptor will be carried
out using standard cloning techniques.

## Key facts

- **NIH application ID:** 3177136
- **Project number:** 2R01CA038808-09
- **Recipient organization:** SAINT LOUIS UNIVERSITY
- **SAM.gov UEI:** JNBLLTBTLLD8
- **Principal Investigator:** JUNG S. HUANG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $282,349
- **Award type:** 2
- **Project period:** 1984-12-01 → 1997-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177136

## Citation

> US National Institutes of Health, RePORTER application 3177136, ROLES OF POLYPEPTIDE GROWTH FACTORS IN CELL GROWTH (2R01CA038808-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177136. Licensed CC0.

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